Bacterial Artificial Chromosomes

BAC Library Construction.- Construction of Small Genome BAC Library for Functional and Genomic Applications.- Preparation of BAC Libraries From Bacterial Genomes by In Vitro Packaging.- Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions.- Purification of BAC DNA.- Hybridization-Based Selection of BAC Clones.- Applications of Interspersed Repeat Sequence Polymerase Chain Reaction.- BAC Mapping Using Fluorescence In Situ Hybridization.- High-Throughput BAC Fingerprinting.- BAC End Sequencing.- Radiation Hybrid Mapping With BAC Ends.- Shotgun Library Construction for DNA Sequencing.- Rolling Circle Amplification for Sequencing Templates.- Transposon-Mediated Sequencing.- Pyrosequencing.- Use of Fimers to Eliminate Polymerase Chain Reaction and Primer-Dimer Artifacts and to Increase Yield in BAC-Sequencing Reactions.- Retrofitting BACs and PACs With LoxP Transposons to Generate Nested Deletions.- Preparing Nested-Deletion Template DNA for Field Inversion Gel Electrophoresis Analyses and Position-Specific End Sequencing With Transposon Primers.- BAC Finishing Strategies.- Using the TIGR Assembler in Shotgun Sequencing Projects.- Finishing “Working Draft” BAC Projects by Directed Sequencing With ThermoFidelase and Fimers.- Optimized Multiplex Polymerase Chain Reaction.- Assembly of DNA Sequencing Data.- Sequence Finishing.- Quality Assessment of Finished BAC Sequences.