MERS Coronavirus

<p>Studying Evolutionary Adaptation of MERS-CoV.-&nbsp;Evaluating MERS-CoV Entry Pathways.-&nbsp;Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Spike Protein Proteolytic Processing.-&nbsp;Crystallization and Structural Determination of the Receptor-binding Domain of MERS-CoV Spike Glycoprotein.-&nbsp;Bacterial Artificial Chromosome-based Lambda Red Recombination with the I-SceI Homing Endonuclease for Genetic Alteration of MERS-CoV.-&nbsp;Deducing the Crystal Structure of MERS-CoV Helicase.-&nbsp;Antigen Capture Enzyme-linked Immunosorbent Assay for Detecting Middle East Respiratory Syndrome Coronavirus in Humans.-&nbsp;Quantification of the Middle East Respiratory Syndrome-Coronavirus RNA in Tissues by Quantitative Real-Time RT-PCR.-&nbsp;Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay.-&nbsp;Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera.-&nbsp;Qualitative and Quantitative Determination of MERS-CoV S1-specific Antibodies using ELISA.-&nbsp;Genetically Engineering a Susceptible Mouse Model for MERS-CoV-Induced&nbsp;Acute Respiratory Distress Syndrome.-&nbsp;Development of&nbsp; a Mouse Adapted MERS Coronavirus.-&nbsp;Metabolite, Protein, and Lipid Extraction (MPLEx): A Method that Simultaneously Inactivates Middle East Respiratory Syndrome Coronavirus and Allows Analysis of Multiple Host Cell Components Following Infection.-&nbsp;Evaluation of Activation and Inflammatory Activity of Myeloid Cells DuringPathogenic Human Coronavirus Infection.-&nbsp;Histopathologic Evaluation and Scoring of Viral Lung Infection.</p>

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