Ivana Vancurova,
Yan Zhu
Immune Mediators in Cancer
Methods and Protocols
Gebonden Engels 2020 9781071602461
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.
Specificaties
ISBN13:9781071602461
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US
Lezersrecensies
Wees de eerste die een lezersrecensie schrijft!
Inhoudsopgave
<p>Part I: Detection of Immune Mediators in Cancer Cells and Tissues</p>
<p> </p>
<p>1. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique</p>
<p> Maria Faresjö</p>
<p> </p>
<p>2. Analysis of Circulating HMGB1 in Human Serum</p>
<p> Weiqiang Chen, Guoqiang Bao, Lin Zhao, and Haichao Wang</p>
<p> </p>
<p>3. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus</p>
<p> Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, and Cong-Qiu Chu</p>
<p> </p>
<p>4. Application of Immunohistochemistry in Basic and Clinical Studies</p>
<p> Aihua Li and Dong-Hua Yang</p>
<p> </p>
<p>5. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma</p>
Takaaki Kamatani</p>
<p> </p>
<p>6. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum</p>
<p> Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, and Petr Vodicka</p>
<p> </p>
<p>7. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence</p>
<p> Herui Wang, Ryan L. Pangilinan, and Yan Zhu</p>
<p> </p>
<p>Part II: Cytokine Bioassays</p>
<p> </p>
<p>8. Analysis of IFNg-Induced Migration of Ovarian Cancer Cells</p>
<p> Bijaya Gaire, Mohammad M. Uddin, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>9. Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells</p>
Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, and Ivana Vancurova</p>
<p> </p>
<p>10. Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids</p>
<p> Mohammad M. Uddin, Bijaya Gaire, and Ivana Vancurova</p>
<p> </p>
<p>11. Detection of Ferroptosis by BODIPY™ 581/591 C11</p>
Alejandra M. Martinez, Ahryun Kim, and Wan Seok Yang</p>
<p> </p>
<p>12. Methods for Studying TNFα-Induced Autophagy</p>
<p> Sheyda Najafi, Ehab M. Abo-Ali, and Vikas V. Dukhande</p>
<p> </p>
<p>13. Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting</p>
Andy Qingan Yuan</p>
<p> </p>
<p>14. Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry</p>
<p> Chong Xu and Yibo Yin</p>
<p> </p>
<p>15. Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells</p>
<p> Wenjun Wu, Zhihui Xiao, Deon Buritis, and Vladimir Poltoratsky</p>
<p> </p>
<p>16. Single-Stranded DNA Aptamers against TNF and Their Potential Applications</p>
<p> Shao Tao, Song Pingfang, Xiaowei Zhang, Lingshu Zhang, and Cong-Qiu Chu</p>
<p> </p>
<p>17. Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells</p>
<p> Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad Uddin, Yan Zhu, and Ales Vancura</p>
<p> </p>
<p>Part III: Expression and Regulation of Immune Mediators in Cancer Cells</p>
<p> </p>
<p>18. Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA</p>
<p> Sveta Padmanabhan, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>19. Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells</p>
<p> Sveta Padmanabhan, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>20. Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation</p>
<p> Yue Zou, Sveta Padmanabhan, and Ivana Vancurova</p>
<p> </p>
<p>21. Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer</p>
<p> Ruizhi Wang, Md. Faruk Hossain, Jovan Mirkovic, Samuel Sabzanov, and Matteo Ruggiu</p>
<p> </p>
<p>22. Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells</p>
<p> Julian J. Freen-van Heeren, Benoit P. Nicolet, and Monika C. Wolkers</p>
<p> </p>
<p>23. Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes</p>
<p> Kiersten P. Carter, Jeffrey E. Segall, and Dianne Cox</p>
<p> </p>
<p>24. Optogenetics: Rho GTPases Activated by Light in Living Macrophages</p>
Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, and Louis Hodgson</p>
<p> </p>
<p>Part IV: Bioinformatics and Genome Editing Approaches</p>
<p> </p>
<p>25. High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer</p>
<p> Susumu Rokudai</p>
</p>
<p>26. Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis</p>
<p> Vitalay Fomin and Carol Prives</p>
<p> </p>
<p>27. Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees</p>
<p> Juan C. Santos</p>
</p>
<p>28. Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing</p>
<p> Olivia Hu, Alessandro Provvido, and Yan Zhu</p>
<p> </p>
<p>29. Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9</p>
<p> Zijun Huo, Jian Tu, Dung-Fang Lee, and Ruiying Zhao</p>
<p> </p>
<p>1. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique</p>
<p> Maria Faresjö</p>
<p> </p>
<p>2. Analysis of Circulating HMGB1 in Human Serum</p>
<p> Weiqiang Chen, Guoqiang Bao, Lin Zhao, and Haichao Wang</p>
<p> </p>
<p>3. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus</p>
<p> Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, and Cong-Qiu Chu</p>
<p> </p>
<p>4. Application of Immunohistochemistry in Basic and Clinical Studies</p>
<p> Aihua Li and Dong-Hua Yang</p>
<p> </p>
<p>5. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma</p>
Takaaki Kamatani</p>
<p> </p>
<p>6. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum</p>
<p> Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, and Petr Vodicka</p>
<p> </p>
<p>7. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence</p>
<p> Herui Wang, Ryan L. Pangilinan, and Yan Zhu</p>
<p> </p>
<p>Part II: Cytokine Bioassays</p>
<p> </p>
<p>8. Analysis of IFNg-Induced Migration of Ovarian Cancer Cells</p>
<p> Bijaya Gaire, Mohammad M. Uddin, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>9. Interleukin-8-Induced Invasion Assay in Triple Negative Breast Cancer Cells</p>
Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, and Ivana Vancurova</p>
<p> </p>
<p>10. Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids</p>
<p> Mohammad M. Uddin, Bijaya Gaire, and Ivana Vancurova</p>
<p> </p>
<p>11. Detection of Ferroptosis by BODIPY™ 581/591 C11</p>
Alejandra M. Martinez, Ahryun Kim, and Wan Seok Yang</p>
<p> </p>
<p>12. Methods for Studying TNFα-Induced Autophagy</p>
<p> Sheyda Najafi, Ehab M. Abo-Ali, and Vikas V. Dukhande</p>
<p> </p>
<p>13. Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting</p>
Andy Qingan Yuan</p>
<p> </p>
<p>14. Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry</p>
<p> Chong Xu and Yibo Yin</p>
<p> </p>
<p>15. Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells</p>
<p> Wenjun Wu, Zhihui Xiao, Deon Buritis, and Vladimir Poltoratsky</p>
<p> </p>
<p>16. Single-Stranded DNA Aptamers against TNF and Their Potential Applications</p>
<p> Shao Tao, Song Pingfang, Xiaowei Zhang, Lingshu Zhang, and Cong-Qiu Chu</p>
<p> </p>
<p>17. Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells</p>
<p> Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad Uddin, Yan Zhu, and Ales Vancura</p>
<p> </p>
<p>Part III: Expression and Regulation of Immune Mediators in Cancer Cells</p>
<p> </p>
<p>18. Immunoblotting Analysis of Intracellular PD-L1 Levels in Interferon-g-Treated Ovarian Cancer Cells Stably Transfected with Bcl3 shRNA</p>
<p> Sveta Padmanabhan, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>19. Flow Cytometry Analysis of Surface PD-L1 Expression Induced by IFNg and Romidepsin in Ovarian Cancer Cells</p>
<p> Sveta Padmanabhan, Yue Zou, and Ivana Vancurova</p>
<p> </p>
<p>20. Analysis of PD-L1 Transcriptional Regulation in Ovarian Cancer Cells by Chromatin Immunoprecipitation</p>
<p> Yue Zou, Sveta Padmanabhan, and Ivana Vancurova</p>
<p> </p>
<p>21. Real-Time PCR Assay for the Analysis of Alternative Splicing of Immune Mediators in Cancer</p>
<p> Ruizhi Wang, Md. Faruk Hossain, Jovan Mirkovic, Samuel Sabzanov, and Matteo Ruggiu</p>
<p> </p>
<p>22. Combined Single-Cell Measurement of Cytokine mRNA and Protein in Immune Cells</p>
<p> Julian J. Freen-van Heeren, Benoit P. Nicolet, and Monika C. Wolkers</p>
<p> </p>
<p>23. Microscopic Methods for Analysis of Macrophage-Induced Tunneling Nanotubes</p>
<p> Kiersten P. Carter, Jeffrey E. Segall, and Dianne Cox</p>
<p> </p>
<p>24. Optogenetics: Rho GTPases Activated by Light in Living Macrophages</p>
Maren Hülsemann, Polina V. Verkhusha, Peng Guo, Veronika Miskolci, Dianne Cox, and Louis Hodgson</p>
<p> </p>
<p>Part IV: Bioinformatics and Genome Editing Approaches</p>
<p> </p>
<p>25. High-Throughput RNA interference Screen Targeting Synthetic-Lethal Gain-of-Function of Oncogenic Mutant TP53 in Triple Negative Breast Cancer</p>
<p> Susumu Rokudai</p>
</p>
<p>26. Discovering Transcription Factor Non-Coding RNA Targets Using ChIP-Seq Analysis</p>
<p> Vitalay Fomin and Carol Prives</p>
<p> </p>
<p>27. Phylogenetic Analyses of Chemokine Receptors from Sequence Retrieval to Phylogenetic Trees</p>
<p> Juan C. Santos</p>
</p>
<p>28. Generation of IL17RB Knockout Cell Lines Using CRISPR/Cas9-Based Genome Editing</p>
<p> Olivia Hu, Alessandro Provvido, and Yan Zhu</p>
<p> </p>
<p>29. Engineering Mutation Clones in Mammalian Cells with CRISPR/Cas9</p>
<p> Zijun Huo, Jian Tu, Dung-Fang Lee, and Ruiying Zhao</p>
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