Lectin Purification and Analysis

Methods and Protocols

Gebonden Engels 2020 9781071604298
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This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.

Specificaties

ISBN13:9781071604298
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer US

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Inhoudsopgave

<div>Structural database for Lectins and the UniLectin Web Platform.-&nbsp;Purification of Sugar-Binding Peptides from L-Type Lectins.-&nbsp;Recombinant Expression and Purification of Animal Intracellular L-Type Lectins.-&nbsp;Frontal Affinity Chromatography: A Highly Suitable Retardation Phenomenon-Based research Tool for Analyzing Weak Interactions between Biomolecules.-&nbsp;Metazoan Soluble&nbsp;b-Galactoside-Binding Lectins, Galectins: Methods for Purification, Characterization of their Carbohydrate Binding Specificity, and probing their Ligands.-&nbsp;Expression, S-Nitrosylation, and Measurement of S-Nitrosylation Ratio of Recombinant Galectin-2.-&nbsp;Expression and Purification of Full-Length and Domain-Fragment Recombinant Pentraxin 3 (PTX3) Proteins from Mammalian and Bacterial Cells.-&nbsp;Identification of Siglec Cis-Ligands by Proximity Labeling.-&nbsp;Preparation of Recombinant Siglecs and Identification of their Ligands.-&nbsp;Purification, Quantification, and FunctionalAnalysis of Collectins.-&nbsp;Selectin-Binding Assay by Flow-Cytometry.-&nbsp;Direct Binding Analysis between C-Type Lectins and Glycans Using Immunoglobulin-Receptor Fusion Proteins.-&nbsp;Expression and Characterization of Hyaluronan Binding Protein Involved in Hyaluronan Depolymerization: HYBID, alias KIAA1199 and CEMIP.-&nbsp;Paracoccin: Purification and validation of its Lectin and Enzymatic Properties.-&nbsp;In Vitro Mannosidase Assay of EDEMs: ER Degradation-Enhancing&nbsp;b-Mannosidase-Like Proteins.-&nbsp;Galactose-Specific, Hemolytic Lectin CEL-III from Cucumaria echinata.-&nbsp;Simple and Rapid Detection of Glycoforms by “Lectin Inhibition” Assay.-&nbsp;Glycoform-Specific Visualization in Immunohistochemistry by “Lectin Inhibition”.-&nbsp;Botulinum Hemagglutinin: Critical protein for Adhesion and Absorption of Neurotoxin Complex in Host Intestine.-&nbsp;Functional Analysis of Botulinum Hemagglutinin (HA).-&nbsp;Purification and Functional Characterization of the Effects onCell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels.-&nbsp;Lectin-Type Ubiquitin Ligase Subunits: Fbs Proteins and their Applications for Use.-&nbsp;F-Type Lectins: Structure, Function, and Evolution.-&nbsp;Purification and Biochemical Characterization of Selected F-Type Lectins.-&nbsp;LecA (PA-IL): A Galactose-Binding Lectin from Pseudomonas aeruginosa.-&nbsp;Glycan Recognition and Application of P-Type Lectins.-&nbsp;Purification and Assays of Tachylectin-5.-&nbsp;Preparation of Soluble Malectin and its Tetramer.-&nbsp;Calnexin/Calreticulin and Assays Related to N-Glycoprotein Folding In Vitro.-&nbsp;Purification and Assays of Tachylectin-2.-&nbsp;Purification and Assays of Tachycitin.-&nbsp;Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host.-&nbsp;ZG16p, An Animal Homologue of Plant β-Prism Fold Lectins: Purification Methods of Natural and Recombinant ZG16p and Inhibition Assay of CancerCell Growth using ZG16p.-&nbsp;ArtinM: Purification and Evaluation of Biological Activities.-&nbsp;Bacterial Expression of Rhamnose-Binding Lectin from Catfish Eggs.-&nbsp;A Bioassay for Determining Symbiotic Zooxanthellae Shape Control Using Lectin SLL-2 from the Octocoral Sinularia lochmodes.-&nbsp;MIC4 from Toxoplasma gondii: A Lectin Acting as a Toll-Like Receptor Agonist.-&nbsp;Production and Characterization of MIC1: A Lectin from Toxoplasma gondii.-&nbsp;Affinity Labeling and Purification of Plant Chitin-Binding LysM-Receptor with Chitin Octasaccharide Derivatives.-&nbsp;Purification of GNA-Related Lectins from Natural Sources.-&nbsp;Expression, Purification, and Applications of the Recombinant Lectin PVL from Psathyrella velutina&nbsp; Specific for Terminal N-Acetyl-Glucosamine.-&nbsp;Yeast Flocculin: Methods for Quantitative Analysis of Flocculation in Yeast Cells.-&nbsp;Pleurotus cornucoiae Mycelial Lectin (PCL-M): Purification and Detection of the Acitivty on Mycelial Surface.-&nbsp;Expression and Purification of a Human Pluripotent Stem Cell-Specific Lectin Probe, rBC2LCN.-&nbsp;Preparation of Fluorescent Recombinant Shiga Toxin-B-Subunit and its Application to Flow Cytometry.-&nbsp;LecB, A High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa.-&nbsp;Sialoglycovirology of Lectins: Sialyl Glycan Binding of Enveloped and Non-Enveloped Viruses.-&nbsp;Hemagglutinin Inhibitors are Potential Future Anti-Influenza Drugs for Mono- and Combination-Therapies.-&nbsp;Preparation and Detection of Glycan Binding Activity of Influenza Virus.-&nbsp;Screening for Components/Compounds with Anti-Rotavirus Activity: Detection of Interaction between Viral Spike Proteins and Glycans.-&nbsp;ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens.-&nbsp;FAM3B/PANDER-Like Carbohydrate-Binding Domain in a Glycosyltransferase, POMGNT1.-&nbsp;Mannose-Specific Oyster Lectin CGL1.-&nbsp;Receptor Binding Assays of Enterovirus D68.-&nbsp;Large-Scale Expression and Purification of Mumps Virus Hemagglutinin-Neuraminidase for Structural Analyses and Glycan-Binding Assays.-&nbsp;Purification of AJLec: A Novel Galactose-Specific Lectin, from the Sea Anemone Anthopleura japonica.-&nbsp;Annexin Lectins: Ca2+-Dependent Heparin-Binding Activity, Phosphatidylserine-Binding Activity, and Anticoagulant Activity.-&nbsp;Expression, Purification, and Functional Characterization of Tectonin 2 from Laccaria bicolor: A Six-Bladed Beta-Propeller Lectin Specific for O-Methylated Glycans.-&nbsp;The OAAH Family: Anti-Influenza Virus Lectins.</div><div><br></div>

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