Corneal Regeneration

<p>Isolation and Culture of Corneal Stromal Stem Cells.-&nbsp;In vitro Expansion of Corneal Endothelial Cells for Transplantation.-&nbsp;Primary Culture of Cornea-Limbal Epithelial Cells In Vitro.-&nbsp;Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant.-&nbsp;A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture.-&nbsp;Gene Editing for Corneal Stromal Regeneration.-&nbsp;Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery.-The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea.-&nbsp;Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding.-&nbsp;Preparation of Dried Amniotic Membrane for Corneal Repair.-&nbsp;Fabrication of Corneal Extracellular Matrix Derived Hydrogels.-&nbsp;Synthesis and Application of Collagens for Assembling a Corneal Implant.-&nbsp;Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts.-&nbsp;Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration.-&nbsp;The use of Animal Models to Assess Engineered Corneal Tissue.-&nbsp;X-ray Diffraction Imaging of Corneal Ultrastructure.</p>

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