Vaccine Delivery Technology

<div>Vaccine Delivery and Immune Response Basics.-&nbsp;Isolating Pathogen-Specific Human Monoclonal Antibodies (hmAbs) using Bacterial Whole Cells as Molecular Probes.-&nbsp;Use of Chlamydial Elementary Bodies as probes to Isolate Pathogen-Specific Human Monoclonal Antibodies.-&nbsp;Computational Antigen Discovery for Eukaryotic Pathogens using Vacceed.-&nbsp;Antigen Discovery in Bacterial Pan-Proteomes.-&nbsp;Alphavirus-Based Antigen Preparation.-&nbsp;Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.-&nbsp;Bioreactor-Based Antigen Production Process using the Baculovirus Expression Vector System.-&nbsp;High-Throughput Process Development for the Chromatographic Purification of Viral Antigens.-&nbsp;Production of Zika Virus Virus-Like Particles.-&nbsp;Biosynthesis of Glycoconjugate Virus-Like Particles (VLPs).-&nbsp;Up- and Downstream Processes for Viral Nanoplaxes as Vaccines.-&nbsp;Whole-Cell Vaccine Preparation: Options and Perspectives.-&nbsp;O-AntigenExtraction, Purification, and Chemical Conjugation to a Carrier Protein.-&nbsp;Oligosaccharide Antigen Conjugation to Carrier Proteins to Formulate Glycoconjugate Vaccines.-&nbsp;Exploitation of Capsule Polymerases for the Enzymatic Synthesis of the Polysaccharide Antigens used in Glycoconjugate Vaccines.-&nbsp;Attenuation Methods for Live Vaccines.-&nbsp;Surface Modification of Adenovirus Vector to Improve Immunogenicity and Tropism.-&nbsp;Production of Baculovirus and Stem Cells for Baculovirus-Mediated Gene Transfer into Human Mesenchymal Stem Cells.-&nbsp;Lipofection-Based Delivery of DNA Vaccines.-&nbsp;Flavivirus DNA Vaccine Design and Adjuvant Selection.-&nbsp;Vaccine Delivery with a Detoxified Bacterial Toxin.-&nbsp;Needle-Less or Non-Invasive Delivery Technology.-&nbsp;Lactic Acid Bacteria as Delivery Vehicle for Therapeutics Applications.-&nbsp;A Hybrid Biological-Biomaterial Vector for Antigen Delivery.-&nbsp;Liposomal Dual Delivery of Both Polysaccharide and Protein Antigens.-&nbsp;Thin-Film Freeze-Drying is a Viable Method to Convert Vaccines Containing Aluminum Salts from Liquid to Dry Powder.-&nbsp;The Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.-&nbsp;Methods to Evaluate Immune Cell Recruitment and the Cellular Uptake and Distribution of Antigen following Intramuscular Administration of Vaccine to Mice.-&nbsp;The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot.-&nbsp;The Measurement of Antigen-Specific IgG Titers by Direct ELISA.-&nbsp;Method to Evaluate In Vivo CD8+ T Cell Cytotoxicity in a Murine Model.-&nbsp;Testing Anti-Pneumococcal Antibody Function using Bacteria and Primary Neutrophils.-&nbsp;Viral Infectivity Qunatification and Neutralization Assays using Laser Force Cytology.</div>