RNA Remodeling Proteins

Key Points to Consider when Studying RNA Remodeling by Proteins.- Happy Birthday : 30 Years of RNA Helicases.- Known Inhibitors of RNA Helicases and their Therapeutic Potential.- Identifying RNA Helicase Inhibitors Using Duplex Unwinding Assays.- Thermal Shift Assay for Characterizing the Stability of RNA Helicases and their Interaction with Ligands.- SRCD and FTIR Spectroscopies to Monitor Protein-induced Nucleic Acid Remodeling.- High throughput Protein-Nucleic Acid Interaction Assay Based on Protein Induced Fluorescence Enhancement.- Probing RNA Helicase Conformational Changes by Single-molecule FRET Microscopy.- A Fluorescent Assay to Monitor Ligand-Dependent Closure of the Hexameric Rho Helicase Ring.- A Simple Fluorescence Microplate Assay to Monitor RNA-DNA Hybrid Unwinding by the Bacterial Transcription Termination Factor Rho.- Monitoring Enzymatic RNA G-quadruplex Unwinding Activities by Nuclease Sensitivity and Reverse Transcription Stop Assays.- Using Magnetic Tweezers to Unravel the Mechanism of the G-quadruplex Binding and Unwinding Activities of DHX36 Helicase.- Characterization of the Brr2 RNA Helicase and its Regulation by other Spliceosomal Proteins using Gel-based U4/U6 di-snRNA Binding and Unwinding Assays.- Monitoring Acetylation of the RNA-helicase DDX3X, a Protein Critical for Formation of Stress Granules.- In vivo cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins.- Deciphering the Dynamic Landscape of Transcription-associated mRNP quality Control Components over the Whole Yeast Genome.- Probing the Conformational State of mRNPs using smFISH and SIM.- Probing Transcriptome-wide RNA Structural Changes Dependent on the DEAD-box Helicase Dbp2.- In situ hybridization – Proximity Ligation Assay (ISH-PLA) to Study the Interaction of HIV-1 RNA and Remodeling Proteins.- RNAi Screening to Identify Factors that Control Circular RNA Localization.- A Cell-Free System for Investigating Human MARF1 Endonuclease Activity.- Monitoring eIF4E-dependent Nuclear 3’ end mRNA Cleavage.- An In Vitro Approach To Study RNase III Activities Of Plant RTL Proteins.- Analysis of Bacillus subtilisRibonuclease Activity In Vivo.- Assay of Bacillus subtilis Ribonuclease Activity in vitro.- Protein Pulldown Assays to Monitor the Composition of the Bacterial RNA Degradosome.