Ras Activity and Signaling

<p>Ras Variant Biology and Contributions to Human Disease.-&nbsp;Regulation of the Small GTPase Ras and its Relevance to Human Disease.-&nbsp;Precise Characterization of KRAS4B Proteoforms by Combining Immunoprecipitation with Top-Down Mass Spectrometry.-&nbsp;Absolute Quantitation of GTPase Protein Abundance.-&nbsp;Validation of Isoform- and Mutation-specific RAS Antibodies.-&nbsp;Production and Membrane Binding of N-terminally Acetylated, C-terminally Farnesylated and Carboxymethylated KRAS4b.-&nbsp;Active GTPase Pulldown Protocol.-&nbsp;Methods to Monitor Ras Activation State.-&nbsp;NMR Detection Methods for Profiling RAS Nucleotide Cycling.-&nbsp;Ras Diffusion and Interactions with the Plasma Membrane Measured by FRAP Variations.-&nbsp;Understanding Ras Spatial Cycles through Reaction-Diffusion Simulations.-&nbsp;Super-Resolution Imaging and Spatial Analysis of RAS on Intact Plasma Membrane Sheets.-&nbsp;FLIM-FRET Analysis of Ras Nanoclustering and Membrane-Anchorage.-&nbsp;Assessment of Plasma Membrane Fatty Acid Composition and Fluidity Using Imaging Flow Cytometry.-&nbsp;Spatiotemporal Imaging of Small GTPase Activity using Conformational Sensors for GTPase Activity (COSGA).-&nbsp;Using BioID to Characterize the RAS Interactome.-&nbsp;Probing RAS function with Monobodies.-&nbsp;Detection of Endogenous RASSF1A Interacting Proteins.-&nbsp;Mathematical Modeling to Study KRAS Mutant-Specific Responses to Pathway Inhibition.-&nbsp;A Facile Method to Engineer Mutant Kras Alleles in an Isogenic Cell Background.-&nbsp;RASless MEFs as a Tool to Study RAS-dependent and -independent Functions.-&nbsp;Generation of Patient-derived Colorectal Cancer Organoids for RAS Studies.-&nbsp;Ras-GEF Mouse Models for the Analysis of Ras Biology and Signaling&nbsp;.-&nbsp;Studying Metabolic Abnormalities in the Costello Syndrome HRAS G12V Mouse Model: Isolation of Mouse Embryonic Fibroblasts and Their In Vitro Adipocyte Differentiation.-&nbsp;Dissecting Oncogenic RAS Signalling in Melanoma Development in Genetically Engineered Zebrafish Models.-&nbsp;Ras, Ral, and Rap1 in C. elegans.</p>

<p>&nbsp;<br></p>