Melanoma

Model Systems for the Study of Malignant Melanoma.- Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing.- A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix.- Wound Healing Assay for Melanoma Cell Migration.- A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells.- Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology.- Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry.- Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry.- In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium.- A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells.- An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence.- Evaluating Melanoma Viability and Proliferation in 3D Microenvironments.- Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models.- Enrichment of Melanoma Cancer Stem Cells via Sphere Assays.- Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry.- Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells.- PD-L1 Detection on Circulating Melanoma Cells.- Transcript-Based Detection of Circulating Melanoma Cells.- Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients.- Simultaneous BRAF^V600E Protein and DNA Aberration Detection in Circulating Melanoma Cells Using an Integrated Multi-Molecular Sensor.- Single Cell Analysis of BRAF^V600E and NRAS^Q61R Mutation Status in Melanoma Cell Lines as Method Generation for Circulating Melanoma Cells.- A Rapid Exosome Isolation Using Size Exclusion Chromatography(REIUS) Method for Exosome Isolation from Melanoma Cell Lines.- Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients.- An Immunocapture-Based Assay for Detecting Multiple Antigens in Melanoma-Derived Extracellular Vesicles.- Post-Lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients.- Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins.- Detection of Melanoma Cells in Lymphatic Drainage (LD) after Lymph Nodes Dissection via Nested RT-PCR Analysis of Molecular Melanocytic Markers.- A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue.- PET Imaging of Melanoma Using Melanin-Targeted Probe.- Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis.- Salivary Proteomic Analysis of Canine Oral Melanoma by MALDI-TOF Mass Spectrometry and LC-Mass Spectrometry/Mass Spectrometry.- Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers.- Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma.- Assessment of Cell-Free microRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients’ Blood.- High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0.- Immunotyping and Quantification of Melanoma Tumor–Infiltrating Lymphocytes.- Single Cell Gene Expression, Clonality, and Feature Barcoding of Melanoma Tumor Infiltrating Lymphocytes.- Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma.- Multiplex Immunohistochemistry Analysis of Melanoma Tumor Infiltrating Lymphocytes.- Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures.- The Use of Nanoparticles in the Delivery of Nucleic Acids for Melanoma Treatment.- siRNA Delivery to Melanoma Cells with Cationic Niosomes.- Controlled Delivery of Plasmid DNA to Melanoma Tumors by Gene Electrotransfer.- Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma.- Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy.