Mitochondrial Medicine

<p>Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow.-&nbsp;Preparation of ‘Functional’ Mitochondria – A Challenging Business.-&nbsp;Isolation and Quality Control of Functional Mitochondria.-&nbsp;Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient.-&nbsp;Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues.-&nbsp;Analysis of Mitochondrial Retrograde Signaling In Yeast Model Systems.-&nbsp;Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes.-&nbsp;Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging.-&nbsp;Assessment of Mitochondrial Cell Metabolism By Respiratory Chain Electron Flow Assays.-&nbsp;Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues.-&nbsp;Single Particle-Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses Of Individual Mitochondria.-&nbsp;Investigation of Mitochondrial ADP-ribosylation via Immunofluorescence.-&nbsp;Assessment of Mitochondrial Ca²⁺ Uptake.-&nbsp;Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices.-&nbsp;Evaluation of Mitochondria Content and Function in Live Cells by Multi-color Flow Cytometric Analysis.-&nbsp;Analysis of Mitochondrial Dysfunction during Cell Death.-&nbsp;Modified Blue-Native Gel Approach for Analysis of Respiratory Super-Complexes.-&nbsp;Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane.-&nbsp;Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway.-&nbsp;3D Optical Cryo-Imaging Method: A Novel Approach To Quantify Renal Mitochondrial Bioenergetics Dysfunction.-&nbsp;Simultaneous Quantification of Mitochondrial ATP and ROS Production Using ATP Energy Clamp Methodology.-&nbsp;High-Throughput Image Analysis of Lipid-Droplet-Bound Mitochondria.-&nbsp;Cell Energy Budget Platform for Multi-Parametric Assessment of Cell and Tissue Metabolism.-&nbsp;Fluorescence-based Assay for Measuring OMA1 Activity.-&nbsp;Studying Mitochondrial Network Formation by in vivo and in vitro Reconstitution Assay.-&nbsp;Extraction of Functional Mitochondria Based on the Membrane Stiffness.-&nbsp;A Protocol for Untargeted Metabolomic Analysis: From Sample Preparation to Data Processing.-&nbsp;A Method for Analysis of Nitrotyrosine-Containing Proteins by Immunoblotting Coupled With Mass Spectrometry.-&nbsp;In vivo Visualization And Quantification of Mitochondrial Morphology in C. elegans.-&nbsp;Assessing Impact of Platinum Complexes on Mitochondrial Functions.-&nbsp;In-silico Modeling of the Mitochondrial Pumping Complexes with Markov State Models.-&nbsp;Monitoring the Mitochondrial Presequence Import Pathway in Living Mammalian Cells with a NewMolecular Biosensor.</p>

<p><br></p>