Mitochondrial Regulation

Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.- Determination of Oxidative Phosphorylation Complexes Activities.- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function.- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs).- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes.- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies.- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases.- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria.- Exploring Liver Mitochondrial Function by ¹³C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry.- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD).- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria.- Mitochondrial Regulation Assessment by ¹³C-NMR Isotopomer Analysis.- Assays for Determination of Cellular and Mitochondrial NAD⁺ and NADH Content.- Following the Dynamism of the Mitochondrial Network in T Cells.- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.