Cardiovascular Development

Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method.- Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample.- RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells.- Preparation of Neonatal Rat Papillary Muscles for Contractile Studies.- In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine.- Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells.- Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining.- Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue.- Laser-induced Choroidal Neovascularization in Rats.- Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain.- Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy.- Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues.- Visualization of Fundus Blood Vessels.- Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements.- Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery.- Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus.- Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining.