The Unfolded Protein Response

Measuring cysteine exposure in unfolded proteins with tetraphenylethene maleimide and its analogues.-&nbsp;Fluorescence-based biosensors for the detection of the unfolded protein response.-&nbsp;Indirect methods to measure unfolded proteins in living cells using fluorescent proteins.-&nbsp;A platform technology for monitoring the Unfolded Protein Response.-&nbsp;The use of fluorescent protein fusions to monitor the unfolded protein response and protein foldase-substrate interactions in plant protoplasts.-&nbsp;High-throughput analysis of protein turnover with tandem fluorescent protein timers.-&nbsp;Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae.-&nbsp;Determination of protein translation during the activation of the UPR.- Determination of the stability and intracellular (intra-nuclear) targeting and recruitment of pre-HAC1 mRNA in the Saccharomyces cerevisiae during the activation of UPR.- Assays to study IRE1 activation and signaling.-&nbsp;Analysis of XBP1 contribution to hyperosmolarity-induced lipid synthesis.- Integrating an ER stress reporter for monitoring genome wide UPR-ER in budding yeast.-&nbsp;Analyze mouse knockout models of UPR pathway elements.-&nbsp;In vitro stimulation of IRE1a/XBP1-deficient B cells with LPS.-&nbsp;Detection of PERK signaling in the central nervous system.-&nbsp;Methods to study the mitochondrial unfolded protein response (UPRmt) in Caenorhabditis elegans.-&nbsp;Drosophila Unfolded Protein Response (UPR) assays in vitro and in vivo.-&nbsp;Protein preparation for proteomic analysis of the unfolded protein response in Arabidopsis thaliana.-&nbsp;Structure-based drug discovery of IRE1 modulators.- Chemical screening to detect ER stress regulators.<p><br></p>