Fusarium wilt

<p>1.&nbsp; Pure culture and DNA sequence-based identification of Fusarium from symptomatic plants and diverse substrates.-&nbsp;Isolation of high-molecular weight (HMW) DNA from Fusarium oxysporum for long-read sequencing.-&nbsp;Karyotyping of Fusarium oxysporum by pulsed field gel electrophoresis and the germ tube burst method.-&nbsp;RNA-seq data analysis for differential expression.-&nbsp;Quantification and isolation of spontaneous colony growth variants.-&nbsp;Generation of a T-DNA insertion mutant library of Fusarium oxysporum using Agrobacterium tumefaciens.-&nbsp;Targeted gene disruption via CRISPR/Cas9 ribonucleoprotein complexes in Fusarium oxysporum.-&nbsp;CRISPR/Cas9 RNP-mediated gene fusion to assess protein quantification and subcellular localization in Fusarium oxysporum.-&nbsp;In vivo monitoring of cytosolic pH using the ratiometric pH sensor pHluorin.-&nbsp;Multi-pronged investigation of volatile compound-mediated interactions of Fusarium oxysporum with plants, fungi and bacteria.-&nbsp;Isolation of mitochondria from Fusarium species.-&nbsp;In planta gene expression analysis and colonization of Fusarium oxysporum.-&nbsp;Time-lapse imaging of root pathogenesis and fungal proliferation without physically disrupting roots.-&nbsp;High-throughput screening assays to identify plant natural products with antifungal properties against Fusarium oxysporum.-&nbsp;Screening and assessment of pisatin demethylase activity (PDA).-Detection and genotyping of Fusarium oxysporum f. sp. vasinfectum race 4 (VCG0114), the Fusarium wilt pathogen of cotton.</p>