Synaptic Vesicles

<p>Real-time quantitative PCR and fluorescence in situ hybridization for subcellular localization of miRNAs&nbsp;in&nbsp;neurons.-&nbsp;Live-imaging of axonal cargoes in Drosophila brain explants using confocal microscopy.-&nbsp;Whole Endosome Recording of Vesicular&nbsp; Neurotransmitter Transporter Currents.-&nbsp;Quantitative analysis of presynaptic vesicle luminal pH in cultured neurons.-&nbsp;Investigation of synaptic vesicle proteins in rat brain tissue using real-time qPCR.-&nbsp;Mass Synaptometry: Applying Mass Cytometry to Single Synapse Analysis.-&nbsp;A Guide to Analysis of Relative Synaptic Protein Abundance by Quantitative Fluorescent Western Blotting.-&nbsp;Synaptosomes and metamodulation of receptors.-&nbsp;A novel method to monitor GABA loading into synaptic vesicles by combining patch pipette perfusion and intracellular, caged-GABA photolysis in brain slice preparations.-&nbsp;Rapid Isolation of Functional Synaptic Vesicles from Tissues through Cryo-grinding, Ultracentrifugation and Size Exclusion Chromatography.-&nbsp;Isolation of synaptic vesicles from mammalian brain.-&nbsp;Building and Using a Two-Photon Fluorescence Crosscorrelation Spectroscopy Setup including Fluorescence Lifetime Analysis.-&nbsp;Fluorescence Lifetime and Cross-correlation Spectroscopy for Observing Membrane Fusion of Liposome Models Containing Synaptic Proteins.-&nbsp;Synaptic vesicle pool monitoring with synaptopH-Iuorin.-&nbsp;Imaging neuropeptide release at Drosophila neuromuscular junction with a genetically engineered neuropeptide release reporter.-&nbsp;Imaging synaptic glutamate release with two-photon microscopy in organotypic slice cultures.-&nbsp;Dynole 34-2 and Acrylo-Dyn 2-30, novel dynamin GTPase chemical biology probes.-&nbsp;Synthesis of Pthaladyn-29 and Napthalimide-10, GTP site directed dynamin GTPase inhibitors.</p><p><br></p>