DNAzymes

<div>A Protocol for Gold Nanoparticle-Assisted Aptamer Selection for a Small Molecule Porphyrin to Develop DNAzyme.-&nbsp;Selection of Aptamer for N-methyl Mesoporphyrin IX to Develop Porphyrin Metalation DNAzyme.-&nbsp;PCR Methods for the Generation of Catalytic ssDNA.-&nbsp;Design of a DNAzyme: Prediction of mRNA Regions Accessible to a DNAzyme.-&nbsp;Fluorescence-Based Kinetic Measurements for RNA-Cleaving DNAzymes.-&nbsp;Stability and Activity of the 10-23 DNAzyme under Molecular Crowding Conditions.-&nbsp;Quantifying the Number and Affinity of Mn2+-Binding Sites with EPR Spectroscopy.-&nbsp;Obtaining Crystals of Nucleic Acids in Complex with the Protein U1A Using the Soaking Method.-&nbsp;Crystallization and Structural Determination of 8-17 DNAzyme.-&nbsp;Solution NMR Spectroscopy as a Tool to Study DNAzyme Structure and Function.-&nbsp;Molecular Modeling and Simulations of DNA and RNA: DNAzyme as a Model System.-&nbsp;Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer.-&nbsp;Chemical Dual End-Labeling of Large Ribozymes.-&nbsp;Spin Labeling of Long RNAs via Click Reaction and Enzymatic Ligation.-&nbsp;Preparation of Site-Specifically Spin-Labeled RNA by In Vitro Transcription using an Expanded Genetic Alphabet.-&nbsp;PELDOR Measurements on Nitroxide-Labelled Oligonucleotides.-&nbsp;Sensing Metal Ions with Phosphorothioate-Modified DNAzymes.-&nbsp;Enhancement of Activity for Peroxidase-Mimicking DNAzyme by Covalent Attachment of Hemin to G-quadruplex-Forming Oligonucleotide Using Click Chemistry.-&nbsp;Generation of RNA with 2’, 3’-cyclic Phosphates by Deoxyribozyme Cleavage in Frozen Solutions.</div><div><br></div>