Single-Domain Antibodies

<p>&nbsp;Nanobodies: From Serendipitous Discovery of Heavy Chain-Only Antibodies in Camelids to a Wide Range of Useful Applications.-&nbsp;Overview, Generation, and Significance of Variable New Antigen Receptors (VNARs) as a Platform for Drug and Diagnostic Development.-&nbsp;Llama DNA Immunization and Isolation of Functional Single-Domain Antibody Binders.-&nbsp;Preparation of Immune and Synthetic VNAR Libraries as Sources of High-Affinity Binders.-&nbsp;&nbsp;Isolation of Single-Domain Antibodies to Transmembrane Proteins Using Magnetized Yeast Cell Targets.-&nbsp;A Transgenic Heavy Chain IgG Mouse Platform as a Source of High Affinity Fully Human Single-Domain Antibodies for Therapeutic Applications.-&nbsp;Cytoplasmic Production of Nanobodies and Nanobody-Based Reagents by Co-Expression of Sulfhydryl Oxidase and DsbC Isomerase.-&nbsp;Small Scale Secretory VHH Expression in Saccharomyces cerevisiae.-&nbsp;Production of Single-Domain Antibodies in Pichia pastoris.-&nbsp;Production of Designer VHH-Based Antibodies in Plant.-&nbsp;Assessing the Aggregation Propensity of Single-Domain Antibodies Upon Heat-Denaturation Employing the ΔT_m Shift.-&nbsp;Facile Affinity Maturation of Single-Domain Antibodies Using Next-Generation DNA Sequencing.-&nbsp;Engineering pH-Sensitive Single-Domain Antibodies.-&nbsp;Humanization of Camelid Single-Domain Antibodies.-&nbsp;Creation of Multimeric Single-Domain Antibodies Using Bacterial Superglues.-&nbsp;Introducing Cysteines into Nanobodies for Site-Specific Labelling.-&nbsp;Affinity-Guided Site Selective Labelling of Nanobodies With Aldehyde Handles.-&nbsp;Cytoplasmic Expression of Nanobodies with Formylglycine Generating Enzyme Tag and Conversion to a Bio-Orthogonal Aldehyde Group.-&nbsp;Site-Specific Fluorescent Labelling, Single-Step Immunocytochemistry, and Delivery of Nanobodies into Living Cells.-&nbsp;Design and Validation of Site-Specifically Labelled Single-Domain Antibody-Based Tracers forIn Vivo Fluorescence Imaging and Image-Guided Surgery..-&nbsp;Design and Preparation of Photobodies: Light-Activated Single-Domain Antibody Fragments.-&nbsp;Visualizing Filoviral Nucleoproteins Using Nanobodies Fused to the Ascorbate Peroxidase Derivatives APEX2 and dEAPX.-&nbsp;Development of Glypican-2 Targeting Single-Domain Antibody CAR T Cells for Neuroblastoma.-&nbsp;Single-Domain Antibodies for Intracellular Toxin Neutralization.-&nbsp;Generation of Single-Domain Antibody-Based Recombinant Immunotoxins.-&nbsp;X-Ray Crystal Structure Analysis of VHH-Protein Antigen Complexes.-&nbsp;Functionalization of Magnetic Beads with Biotinylated Nanobodies for MALDI-TOF/MS-Based Quantitation of Small Analytes.-&nbsp;Nanobody-Based Assays for the Detection of Environmental and Agricultural Contaminants.-&nbsp;Peptide Tag-Specific Nanobodies for Studying Proteins in Live Cells.-&nbsp;Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology.-&nbsp;Optogenetic Activation of Intracellular Nanobodies.</p>

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