Directed Evolution

<div>Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach.-&nbsp;Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR).-&nbsp;Non-Degenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization.-&nbsp;Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries.-&nbsp;SpeedyGenesXL An Automated, High-Throughput Platform for the Preparation of Bespoke Ultra-Large Variant Libraries for Directed Evolution.-&nbsp;Facile Assembly of Combinatorial Mutagenesis Libraries using Nicking Mutagenesis.-&nbsp;GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes.-&nbsp;Rapid Cloning of Random Mutagenesis Libraries using PTO-QuickStep.-&nbsp;Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs.-&nbsp;Application of Restriction Free (RF) Cloning in Circular Permutation.-&nbsp;Site-Directed Mutagenesis Method Mediated by Cas9.-&nbsp;Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity.-&nbsp;A Screening Method for P450 BM3 Mutant Libraries using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds.-&nbsp;Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method.-&nbsp;Learning Strategies in Protein Directed Evolution.</div><div><br></div>