Regulatory T-Cells

<p>Analysis of the in vivo function of follicular regulatory T (TFR) cells in the regulation of antibody response.- Analysis of peripherally derived Treg in the intestines.- Isolation and analysis of tumor infiltrating Treg.- Induction of peripheral Treg.- Adoptive transfer and bone marrow chimera models to analyse Treg function and differentiation.- Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4⁺ T cells.- Genetic Tools for Analyzing Foxp3⁺ Treg cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping.- Depletion of Treg by the Diphtheria Toxin System.- Analysis of FOXP3 DNA methylation patterns to identify functional FOXP3+ T-Cell subpopulations.- Protein Ubiquitination Assay.- Phenotypic and Functional Studies of Human Treg cell Subpopulations.-&nbsp;In vitro analysis of CTLA-4 mediated transendocytosis by Regulatory T cells.- Isolation and functional characterisation of regulatory CD4+ Tcells from the inflamed joints of patients with rheumatoid arthritis.- A GMP protocol for the manufacture of regulatory T cells for clinical application.- Regulatory T-cell phenotyping using CyTOF.- High-dimensional single-cell profiling of tumor-infiltrating CD4+ regulatory T cells.- Single-cell transcriptome analysis of Treg.</p>