Plant Proteostasis

Observing real-time ubiquitination in high throughput with fluorescence polarization.- Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating-Enzymes and E3 Ubiquitin Ligases.- Immunoprecipitation of Cullin Ring Ligases (CRLs) in Arabidopsis thaliana seedlings.- An in vitro assay to recapitulate hormone-triggered & SCF-mediated protein ubiquitylation.- Analysis of proteasome-associated ubiquitin ligase activity.- Measuring the DUB Activity of Arabidopsis Deubiquitylating enzymes.- SUMO conjugation and SUMO chain formation by plant enzymes.- Kinetic analysis of plant SUMO conjugation machinery.- Expression, purification, and enzymatic analysis of plant SUMO proteases.- Monitoring autophagic flux in the model single-celled microalga Chlamydomonas reinhardtii.- Detection of autophagy in plants by fluorescence microscopy.- Characterization of ATG8-family interactors by Isothermal Titration Calorimetry.- Protocols for studying protein stability in a Arabidopsis protoplast transient expression system.- Relative Protein Lifetime Measurement in Plants using Tandem Fluorescent Protein Timers.- Detection and Quantification of Protein Aggregates In Plants.- Using intrinsic fluorescence to measure protein stability upon thermal and chemical denaturation.- Purification and detection of ubiquitinated plant proteins using Tandem Ubiquitin Binding Entities.- Titanium oxide-based phosphopeptide enrichment from Arabidopsis seedlings.- Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea.- Chromatin Enrichment for Proteomics in Plants (ChEP-P).- Proximity-dependent in vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha.- Tandem mass tag-based phospho-proteomics in plants.- Analysis of peptide hormone maturation and processing specificity using isotope-labeledpeptides.- Improved identification of protease cleavage sites by in-gel reductive dimethylation.- A Pipeline to Monitor Proteasome Homeostasis in Plants.- Bioinformatics tools for exploring the SUMO Gene Network, an update.- Coexpression network constriction and visualization from transcriptomes underlying ER stress responses.- Approaches for the identification of intrinsically disordered protein domains.