Mitochondrial DNA

Isolation of functional mitochondria and pure mtDNA from murine tissues.-&nbsp;Isolating mitochondria, mitoplasts, and mtDNA from cultured mammalian cells.-&nbsp;Coupling differential centrifugation with exonuclease treatment and size exclusion &nbsp;&nbsp;&nbsp;&nbsp;&nbsp; chromatography (DIFSEC) for purification of mtDNA from mammalian cells.-&nbsp;Isolation and Quality Control of Yeast Mitochondria.-&nbsp;Mitochondrial DNA isolation from plants.-&nbsp;Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) nucleoids with &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Multiple Labelling Strategies.-&nbsp;Visualization of mtDNA using FISH.-&nbsp;In situ analysis of mitochondrial DNA synthesis using metabolic labeling coupled to &nbsp;&nbsp;&nbsp; fluorescence microscopy.-&nbsp;Measurement of nucleoid size using STED microscopy.-&nbsp;How to quantify DNA compaction by TFAM with Acoustic Force Spectroscopy and Total &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Internal Reflection Fluorescence microscopy.-&nbsp;Assessing TFAM binding to human mitochondrial DNA.-&nbsp;Identification of proximity interactors of mammalian nucleoid proteins by BioID.-&nbsp;Localization of mitochondrial nucleoids by transmission electron microscopy using the transgenic expression of the mitochondrial helicase TWINKLE and APEX2.-&nbsp;In vitro assays of TWINKLE function.-&nbsp;Rolling Circle Replication and Bypass of Damaged Nucleotides.-&nbsp;Studying mitochondrial nucleic acid synthesis utilizing intact isolated mitochondria.-&nbsp;Functional assessment of mitochondrial DNA maintenance by depletion and repopulation &nbsp;&nbsp;&nbsp; using 2’,3’-dideoxycytidine in cultured cells.-&nbsp;Analysis of mitochondrial DNA replication by two-dimensional agarose gel electrophoresis.-&nbsp;Quantitative analysis of nucleoside triphosphate pools in mouse muscle using hydrophilic &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; interaction liquid chromatography coupled with tandem mass spectrometry detection.-&nbsp;Detection of UV-Induced Deletions in Mitochondrial DNA.-&nbsp;Determination of the ribonucleotide content of mtDNA using alkaline gels.-&nbsp;5´-end mapping in human mitochondrial DNA.-&nbsp;Manipulation of murine mitochondrial DNA heteroplasmy with mtZFNs.-&nbsp;Biolistic transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae &nbsp;&nbsp;&nbsp;&nbsp; mitochondria.-&nbsp;A method for precisely identifying modifications to plant mitochondrial genomes by &nbsp;&nbsp;&nbsp; mitoTALENs.-&nbsp;Mitochondrial DNA sequencing and heteroplasmy quantification by Next Generation &nbsp;&nbsp;&nbsp; Sequencing.-&nbsp;Genomic strategies in mitochondrial diagnostics.-&nbsp;Mitochondrial DNA enrichment for sensitive next-generation sequencing.-&nbsp;Single cell analysis of mitochondrial DNA deletions.<br> <br>