DNA Manipulation and Analysis

Classical Recombinant DNA Cloning.-&nbsp;A Sequence and Ligation-Independent Cloning (SLIC) Procedure for the Insertion of Genes into a Plasmid Vector.-&nbsp;Molecular cloning using in vivo DNA assembly.-&nbsp;Assembling multiple fragments: The Gibson Assembly.-&nbsp;TA cloning approaches to cloning DNA with damaged ends DNA.-&nbsp;PCR-based Assembly of Gene Sequences by Thermodynamically Balanced Inside-Out (TBIO) Gene Synthesis.-&nbsp;Random Mutagenesis by PCR.-&nbsp;In vitro Site Directed Mutagenesis.-&nbsp;Xenopus transgenesis using the pGateway system.-&nbsp;CRISPR/Cas9 gene disruption studies in F0 Xenopus tadpoles: understanding development and disease in the frog.-&nbsp;A CRISPR/Cas based method for precise DNA integration in Xenopus laevis oocytes followed by intracytoplasmic sperm injection (ICSI) fertilisation.-&nbsp;A Lambda-Exonuclease SELEX Method for Generating Aptamers to Bacterial Targets. -Generation of functional-RNA arrays by in vitrotranscription and in situ RNA capture for the detection of RNA-RNA interactions.-&nbsp;Chemical synthesis of oligonucelotide sequences: Phosphoramidite chemistry.-&nbsp;Low Throughput Direct Cycle Sequencing of Polymerase Chain Reaction (PCR) Products.-&nbsp;Next generation Sequencing for mixed samples.-&nbsp;Ethics, Legality and Safety for Geneticists.<p><br></p>