Polycomb Group Proteins

<div>Analyzing the Genome-Wide Distribution of Histone Marks by CUT&Tag in Drosophila Embryos.-&nbsp;Chromatin Preparation and Chromatin Immuno-Precipitation from Drosophila Heads.-&nbsp;Detecting Cell Compartment Specific PRC2-RNA Interactions via UV-RIP.-&nbsp;Chromosome Conformation Capture Followed by Genome-Wide Sequencing (Hi-C) in Drosophila Embryos.-&nbsp;Physics-Based Polymer Models to Probe Chromosome Structure in Single Molecules.-&nbsp;Co-Immunoprecipitation (Co-IP) in Mammalian Cells.-&nbsp;Site Directed Mutagenesis Protocol to Determine the Role of Amino Acid Residues in Polycomb Group (PcG) Protein Function.-&nbsp;Isolation of Chromatin Proteins by Genome Capture.-&nbsp;Dynamic Interactome of PRC2-EZH1 Complex Using Tandem-Affinity Purification and Quantitative Mass Spectrometry.-&nbsp;Replication Timing of Gene Loci in Different Cell Cycle Phases.-&nbsp;Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.-&nbsp;Segmentation, 3D Reconstruction, and Analysis of PcG Proteins in Fluorescence Microscopy Images in Different Cell Culture Conditions.-&nbsp;STORM Microscopy and Cluster Analysis for PcG Studies.-&nbsp;Quantitative Analysis of PcG-Associated Condensates by Stochastic Optical Reconstruction Microscopy (STORM).-&nbsp;Immunoelectron Microscopy Methods.-&nbsp;Differentiation of hPSCs to Study PRC2 Role in Cell-Fate Specification and Neurodevelopment.-&nbsp;Analysis of Polycomb Epigenetic Marks in HeLa Spheroids.-&nbsp;Establishment and Maintenance of Human CRC-Derived Organoids for PcGs Studies.</div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div>