Kidney Research

<p>Restoration of podocyte phenotype in culture.-&nbsp;Protocols for the propagation, culture and characterization of kidney fibroblasts.-&nbsp;Isolation of rat glomeruli and propagation of mesangial cells to study the kidney in health and disease.-&nbsp;In vitro models to evaluate molecular permeability of the kidney filtration barrier.-&nbsp;Generating human glomeruli from pluripotent stem cells for disease modelling and drug screening.-&nbsp;Large-scale production of kidney organoids from human pluripotent stem cells.-&nbsp;Construction of a multitubular perfusable kidney-on-chip for the study of renal disease.-&nbsp;A “kidney-on-the-chip” model composed of primary human tubular, endothelial, and white blood cells.-&nbsp;Standardized protocol for the preparation of precision-cut kidney slices:&nbsp; a translational model of renal fibrosis.-&nbsp;Culture of three-dimensional Madin-Darby Canine Kidney (MDCK) cysts for in vitro drug testing in polycystic kidney disease.-&nbsp;Zebrafishmodel to study podocyte function within the glomerular filtration barrier.-&nbsp;Magnetic Resonance Imaging (MRI) analysis of tissue sodium concentration in chronic kidney disease.-&nbsp;Determination of interstitial collagen deposition and related topography using the second harmonic generation-based Histoindex platform.-&nbsp;Quantitative imaging of podocyte foot processes in the kidney using confocal and STED microscopy.-&nbsp;Synthesis and expression of a targeted, ferritin-based tracer for PET imaging of kidney glomeruli.-&nbsp;Multiplex in situ hybridization in the study of acute kidney injury.-&nbsp;Spatial transcriptomics in kidney tissue.-&nbsp;Assessment of kidney mitochondrial function by high-resolution respirometry, transmission electron microscopy and histological techniques.-&nbsp;Transdermal measurement of glomerular filtration rate in preclinical research.-&nbsp;The BioHybrid Assay – a novel method for determining calcification propensity.-&nbsp;Quantification of calciprotein particles (CPPs) in serum/plasma samples using a fluorescent bisphosphonate.-&nbsp;Monitoring of Rho GTPase Activity in Podocytes.</p>

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