Non-Ribosomal Peptide Biosynthesis and Engineering

The Assembly-line Enzymology of Nonribosomal Peptide Biosynthesis.-&nbsp;Structural Studies of Modular Nonribosomal Peptide Synthetases.-&nbsp;Using NMR Titration to Study Protein-Protein Interactions in AcpP Dependent FAS-II.-&nbsp;Chemoproteomic Profiling of Adenylation Domain Functions in Gramicidin S-producing Nonribosomal Peptide Synthetases.-&nbsp;In Vitro Biochemical Characterization of Excised Macrocyclizing Thioesterase Domains from Non-Ribosomal Peptide Synthetases.-&nbsp;Chemo-Enzymatic Synthesis of Non-Ribosomal Macrolactams by a Penicillin-binding Protein-type Thioesterase.-&nbsp;Directed Evolution of the BpsA Carrier Protein Domain for Recognition by Non-cognate 4’-Phosphopantetheinyl Transferases to Enable Inhibitor Screening.-&nbsp;Unraveling Structural Information of Multi-Domain Nonribosomal Peptide Synthetases by Using Photo-Crosslinking Analysis with Genetic Code Expansion.-&nbsp;A Chemoenzymatic Approach to Investigate Cytochrome P450 Crosslinking in Glycopeptide Antibiotic Biosynthesis.-&nbsp;Cross-Linking of the Nonribosomal Peptide Synthetase Adenylation Domain with a Carrier Protein Using a Pantetheine-Type Probe.-&nbsp;A Practical Guideline to Engineering Non-Ribosomal Peptide Synthetases.-&nbsp;Probing Substrate-Loaded Carrier Proteins by Nuclear Magnetic Resonance.-&nbsp;Ribosomal Synthesis of Peptides Bearing Noncanonical Backbone Structures via Chemical posttranslational modifications.-&nbsp;Thioester Capture Strategy for the Identification of Nonribosomal Peptide and Polyketide Intermediates.-&nbsp;Chemical Labeling of Protein 4′-Phosphopantetheinylation in Surfactin-Producing Nonribosomal Peptide Synthetases.-&nbsp;Norine: Bioinformatics Methods and Tools for the Characterization of Newly Discovered NonRibosomal Peptides.<p><br></p>