NLR Proteins

Canonical Inflammasomes.- Inflammasome-independent roles of NLR and ALR family members.- Measuring IL-1β processing by bioluminescence sensors: using a Bioluminescence Resonance Energy Transfer biosensor.- Methods to measure NLR oligomerization I: size exclusion chromatography, co-immunoprecipitation and cross-linking.- Measuring NLR oligomerization II: detection of ASC speck formation by confocal microscopy and immunofluorescence.- Measuring NLR oligomerization III: detection of NLRP3 and NLRC4 complex by bioluminescence resonance energy transfer.- Method to measure ubiquitinization of NLRs.- Methods to study NLR in human blood cells.- Non-canonical NLRP3 inflammasome activation: standard protocols.- Pyroptosis induction and visualization at the single cell level using optogenetics.- Determination of gasdermin pores.- Methods to activate the NLRP3 inflammasome.- Quantifying cell death induced by the NLRC4 inflammasome.- Methods to activate the NLRP1 inflammasome.- Methods to study inflammasome activation in the central nervous system: immunoblotting and immunohistochemistry.- Inflammasome activation in human macrophages: IL-1β cleavage detection by fully automated capillary-based immunoassay.- Assessing the ATP binding ability of NLRP3 from cell lysates by a pull-down assay.- Theoretical 3D-Modeling of NLRP3 inflammasome complex.- A knock-in mouse model of cryopyrin-associated periodic syndromes.