Deadenylation

The reconstitution of human CCR4-NOT from purified proteins and an assay of its deadenylation activity.- In vitro reconstitution of the Drosophila melanogaster CCR4-NOT complex to assay deadenylation.- Analysis of human mRNA deadenylation complexes via high-resolution gel electrophoresis.- Quantitative biochemical analysis of deadenylase enzymes using fluorescence and chemiluminescence-based assays.- A FRET-based assay to quantify enzymatic rates and explore the mechanisms of RNA deadenylases in heterogeneous environments.- Measuring Poly-Adenosine Tail Length of RNAs by High Resolution Northern Blotting Coupled with RNase H Cleavage.- An RNA-ligation-based RACE-PAT assay to monitor poly(A) tail length of mRNAs of interest.- Dissecting the role of the Ccr4-Not deadenylase complex in pluripotency and differentiation.- Measuring proximity-mediated function of mRNA regulatory proteins by engineered tethering.-Tethered mRNA Amplifier: A NovelApproach to Increase Protein Expression.- RNA binding protein-mediated mRNA deadenylation in mammalian cell extracts.- Transcriptome-wide analysis of mRNA adenylation status in yeast using nanopore sequencing.- Sequencing of transcriptome-wide poly(A) tails by PAIso-seq.- Nano3′RACE: a method to analyze poly(A) tail length and nucleotide additions at the 3′ extremity of selected mRNAs using nanopore sequencing.- Quantification of poly(A) tail length and terminal modifications using direct RNA sequencing.- Differential poly(A) tail length analysis using nanopore sequencing.- Single-molecule poly(A) tail sequencing (SM-PATseq) using the PacBio platform.- Mathematical models for the poly(A) tail shortening process.