Bacterial Regulatory RNA

RNA extraction from Gram-positive bacteria membrane vesicles using a polymer-based precipitation method.-&nbsp;Extraction and purification of outer membrane vesicles and their associated RNAs.-&nbsp;Analysis of phage regulatory RNAs: sequencing library construction from the fraction of small prokaryotic RNAs less than 50 nucleotides in length.-&nbsp;Discovering Novel Bacterial Small RNA by RNA-seq Analysis Toolkit ANNOgesic.-&nbsp;Ribosome profiling methods adapted to the study of RNA-dependent translation regulation in Staphylococcus aureus.-&nbsp;CRISPR interference-based functional small RNA genomics.-&nbsp;Investigation of sRNA-mRNA interactions in Bacillus subtilis in vivo.-&nbsp;In vitro methods for the investigation of sRNA-mRNA interactions in Bacillus subtilis.-&nbsp;RNA double helix hybridization measured by fluorescence correlation spectroscopy.-&nbsp;New perspectives on cross talks between bacterial regulatory RNAs from outer membrane vesicles and eukaryotic cells.-&nbsp;Experimental validation of RNA-RNA interactions by Electrophoretic Mobility Shift Assay.-&nbsp;Dynamics and function of sRNA/mRNAs under the scrutiny of computational simulation methods.-Analysis of sRNAs and their mRNA targets in Sinorhizobium meliloti: focus on half-live determinationEvaluation of 5'-end phosphorylation for small RNA stability and target regulation in vivo.-&nbsp;In-gel cyanoethylation for pseudouridines mass spectrometry detection of bacterial regulatory RNA.-&nbsp;Directed screening for sRNA targets in E. coli using a plasmid library.-&nbsp;Defining Bacterial RNA-RNA Interactomes Using CLASH.-&nbsp;Global identification of RNA-binding proteins in bacteria.-&nbsp;An integrated affinity chromatography-based approach to unravel the sRNA interactome in nitrogen-fixing rhizobia.-&nbsp;sRNA structural modeling based on NMR data.-&nbsp;Circular and linear dichroism for the analysis of small noncoding RNA properties.<p><br></p>