The Plant Endoplasmic Reticulum

<p>&nbsp;Make it shine: Labelling the ER for light and fluorescence microscopy.-&nbsp;Electron microscopy techniques for 3D plant ER imaging.-&nbsp;Studying plant ER-PM contact site localised proteins using microscopy.-&nbsp;Preparation and imaging of specialised ER using super-resolution and TEM techniques.-&nbsp;Quantitation of ER morphology and dynamics.-&nbsp;Long-term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination.-&nbsp;Dancing with the stars: Using image analysis to study the choreography of the endoplasmic reticulum and its partners and of movement within its tubules.-&nbsp;Preparation of highly enriched ER membranes using free-flow electrophoresis.-Preparation of ER microsomes from Arabidopsis thaliana.-&nbsp;ER membrane lipid composition and metabolism: lipidomic analysis.-&nbsp;2in1 vectors improve in planta BiFC and FRET analysis.-&nbsp;Immunoprecipitation and FRET-FLIM to determine metabolons on the plant ER.-&nbsp;Using optical tweezers combined with total internal reflection microscopy to study interactions between the ER and Golgi in plant cells.-&nbsp;Protein biosynthesis and maturation in the ER.-&nbsp;ER membrane Protein Interactions using the Split-Ubiquitin System (SUS).-&nbsp;Analysis of protein glycosylation in the ER.-&nbsp;Unfolded Protein Response in Arabidopsis.-&nbsp;Imaging the ER and endomembrane system in cereal endosperm.-&nbsp;Multi-omics resources for understanding gene regulation in response to ER stress in plants.-&nbsp;Variable angle epifluorescence microscopy for single particle tracking in the plant ER.-&nbsp;Use of super resolution live cell imaging to distinguish endoplasmic reticulum - nuclear envelope subcellular localisation.-&nbsp;Using ER-targeted photoconvertible fluorescent proteins in living plant cells.-&nbsp;In vivo analysis of ER-associated protein degradation and ubiquitination in Arabidopsis thaliana.-&nbsp;Overexpression of endoplasmic reticulum proteins from Arabidopsis thaliana in baculovirus.-&nbsp;Observing ER dynamics over long timescales using light sheet fluorescence microscopy.-&nbsp;Gene stacking and stoichiometric expression of ER-targeted constructs using “2A” self-cleaving peptides.-&nbsp;Automated Segmentation and 3D Reconstruction of Different Membranes from Confocal Z-stacks.-&nbsp;Visualizing orientation and topology of ER membrane proteins in planta.-&nbsp;Organ specific microsomes from dark grown hypocotyls of Arabidopsis thaliana.-&nbsp;Studying secretory protein synthesis in Nicotiana benthamiana protoplasts.-&nbsp;Analyzing photoactivation with diffusion models to study transport in the Endoplasmic Reticulum network.&nbsp;</p>

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