Mammalian Synthetic Systems

A directed evolution protocol for engineering minimal transcription factors, based on CIS display.- Set-up and applications of Modular Protein Expression Toolboxes (MoPET) for mammalian systems.- Coiled-coiled interaction toolbox for engineering mammalian cells.- A mammalian-based synthetic biology toolbox to engineer membrane-membrane interfaces.- Engineered protease-responsive RNA Binding Proteins (RBPs) to expand the toolbox of synthetic circuits in mammalian cells.- Mechanistic model-driven biodesign in mammalian synthetic biology.- Realizing Antithetic Integral Feedback Control in Mammalian Cells.- A computational modeling approach for the design of genetic control systems that respond to transcriptional activity.- Flux Balance Analysis of Mammalian Cell Systems.- Multiplex functional characterization of protein variant libraries in mammalian cells with single-copy genomic integration and high throughput DNA sequencing.- Flow Cytometry Quantification of Transient Transfections in Mammalian CellsRNA switches using Cas proteins.- Multiplexed transactivation of mammalian cells using dFnCas12a-VPR.- Anti-CRISPR Proteins and their Application to Control CRISPR-effectors in Mammalian Systems.- Post-translational Remote Control Mediated by Physical Inducers for Rapid Protein Release in Engineered Mammalian Cells.- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells.- Detection of microRNAs Using Synthetic Toehold Switch in Mammalian Cells.- High-throughput spectroscopic analysis of mRNA capping level.- In vitro generation of megakaryocytes from engineered mouse embryonic stem cells.- Preparation of chromobodies for the detection of cell surface epitopes.