KRAS

KRAS as a therapeutic target.-&nbsp;The abundance of KRAS and RAS gene mutations in cancer.-&nbsp;Production of isotopically labeled KRAS4b.-&nbsp;Nucleotide Exchange on RAS Proteins using Hydrolysable and Non-Hydrolysable Nucleotides.-&nbsp;Crystallographic studies of KRAS in Complex with Small Molecules and RAS-binding Proteins.-&nbsp;Considerations around Structure-Based Drug Discovery for KRAS using DOCK.-&nbsp;Measurement of KRAS GTPase activity.-&nbsp;Affinity Measurement of Non-covalent Interactions of the covalent KRAS G12C GDP inhibitor MRTX849 to RAS Isoforms using Surface Plasmon Resonance.-&nbsp;1D and 2D NMR for KRAS:ligand binding.-&nbsp;Luminescence based techniques for KRAS thermal stability monitoring.-&nbsp;MALDI-TOF mass spectrometry-based assay for measuring covalent target engagement of KRAS G12C inhibitors.-&nbsp;KRAS4b:RAF-1 Homogenous Time-Resolved Fluorescence Resonance Energy Transfer Assay for Drug Discovery.-&nbsp;Biophysical characterization of RAS-SOS Complexes by Native Mass Spectrometry.-&nbsp;Profiling complex RAS-effector interactions using NMR spectroscopy.-&nbsp;Probing RAS Function Using Monobody and NanoBiT Technologies.-&nbsp;Analysis of the guanine nucleotide-bound state of KRAS by ion-pair reversed-phase high-performance liquid chromatography.-&nbsp;Real-time monitoring of RAS activity using in vitro and in-cell NMR spectroscopy.-&nbsp;Studying RAS Interactions in live cells with BRET.-&nbsp;FLIM-FRET Protein-Protein Interaction Assay.-&nbsp;High-throughput cell-based screening of small molecule KRAS signaling inhibitors using a homogeneous time resolved fluorescence (HTRF) assay.-&nbsp;A method to conditionally measure target engagement at intracellular RAS and RAF complexes.-&nbsp;FLAG-KRAS4B as a model system for KRAS4B proteoform and PTM evaluation by mass spectrometry .-&nbsp;Creation of an isogenic H/N/KRAS-less mouse embryonic fibroblast cell line panel derived from a size sorted diploid clone.-&nbsp;Quality Control ofan Isogenic H/N/KRAS-less Mouse Embryonic Fibroblast Cell Line Panel.-&nbsp;A Facile Method to Append a Bio-ID Tag to Endogenous Mutant Kras Alleles.<p></p>

<br>