Iron Metabolism

Iron Homeostatic Regulation in Saccharomyces cerevisiae: Introduction to a Computational Modeling Method.- ICP-MS Analysis of Iron from Biological Samples.- Sample Preparation for X-Ray Fluorescence Microscopy of Iron Distribution in Biological Specimen.- Proximity Ligation Assay for the Analysis of Iron Protein Interactions in the Nucleus.- Fluorescence-Based Proteoliposome Methods to Monitor Redox-Active Transition Metal Transmembrane Translocation by Metal Transporters.- Metal Uptake by Mitochondrial Carrier Family Proteins Using Lactococcus lactis.- Methods for Cultivation of Bacteroides thetaiotaomicron and Analysis of Heme Metabolism by Mass Spectrometry and Spectroscopic Approaches.- Heme o Isolation and Analysis Using Heme o Synthase Heterologously Expressed in Escherichia coli.- Fluorometric Methods to Measure Bioavailable and Total Heme.- Recombinant Biogenesis and Analysis of Cytochrome c Species.- Ultra Performance Liquid Chromatography (UPLC) Analysis of Heme Biosynthesis Intermediates.- Radiolabeling Heme and Porphyrin with 14C-Glycine or 14C -Aminolevulinic Acid.- Heterologous Expression and Purification of Eukaryotic ALA Synthase from E. coli.- Direct Spectroscopic Ferrochelatase Assay.- Reverse Thiol Trapping Approach to Assess the Thiol Status of Metal-Binding Mitochondrial Proteins.- A Chemoproteomic Approach to Monitor Native Iron-Sulfur Cluster Binding.