Biological Macromolecule Engineering Technologies

High Level Protein Expression in Pseudomonas putida Using the Phage-Based pPUT Expression System.- New-to-Nature Photoenzymatic Catalysis.- CRISPR-Hybrid: Intracellular Selection of CRISPR-Associated Aptamers in Bacteria.- Designing and Engineering Enzymes for New-to-Nature Photobiocatalysis.- A Yeast Two-Hybrid Assay to Develop Optogenetic Tools for Controlling Protein-Protein Interactions.- CRISPR-Engineered Bacteriophage T4 for Foot-and-Mouth Disease Nanoparticle Vaccine Development.- Endoskeleton-Enhanced Virus-Like Particles (VLPs): Internal Cross-Linking of Physalis Mottle Virus and Stability Tests.- An Integrated Workflow for Phage Selection, Amplification, and Surface Modification.- CRISPR-Cpf1-Mediated T4 Phage Genome Editing for One-Step In Vivo Display of Heterologous Protein.- Methods for Applying Prime Editing and Inverse Prime Editing in Mammalian Cell Culture.- Constructing Drive-and-Process (DAP) CRISPR Guide RNA Arrays for Multiplexed Base- and Prime-Editing.- Production of Cell Models with Differential Zygosity Using CRISPR Base Editors.- Phage-Assisted Continuous Selection of Bioactive Cyclic Peptides.- Targeted Transition Mutations in Phage-Assisted Evolution.- Phage Display In Vivo: Protocols for Identifying Brain-Targeting Peptides in Mouse Models of Neurodegeneration.- Generation of Diversely Functionalized DNA Libraries Using Ligase-Catalyzed Oligonucleotide Polymerization (LOOPER).- Solid Phase Chemical Synthesis of RNA.- Fluorescence-Based Assays to Evaluate the Functionality of Phage-Derived Regulatory Elements for Applications in Synthetic Biology.- Using peCHYRON to Record Mammalian Cells’ History of Chemical Exposure into Their DNA.