Chlamydia trachomatis

Monitoring Chlamydia trachomatis Development in Polarized Endocervical Epithelial Cells.- Chlamydia trachomatis Infection in a Microphysiologic Cell Culture Model.- Quantifying Chlamydia in the Mouse Gastrointestinal Tract.- Quantification of Human T Cell Responses to Chlamydia trachomatis: Intracellular Cytokine Staining (ICS) and Activation-Induced Marker (AIM) Assays.- Live Cell Imaging of Chlamydia Inclusion Membrane Proteins Using Genetically-Encoded Antibodies.- Live Imaging of Ions in Cells Infected with Chlamydia trachomatis.- Labeling and Detection of Peptidoglycan in Chlamydia and Chlamydia-Related Organisms Utilizing D-Alanine Analogs.- High-Resolution Detection of Chlamydia Proteins by Expansion Microscopy.- High-Resolution Detection of Sphingolipids during Chlamydia trachomatis Infection via Expansion Microscopy.- Genome-Wide Quantification of Protein-DNA Interactions in Chlamydia Using ChIP-Seq (Chromatin Immunoprecipitation/Deep Sequencing).- Analysis of Spatial Gene Expression in Chlamydia Using Fluorescent In Situ Hybridization.- Analysis of Chlamydia trachomatis Gene Function via Controlled Up- or Down-Regulation.- Calcium Chloride-Mediated Transformation of Chlamydia trachomatis and Other Chlamydia spp.- Generation of Chromosomal Deletions in Chlamydia trachomatis.- Chromosome Editing Using the Lambda Red Recombinase System in Chlamydia trachomatis and Chlamydia muridarum.- Construction of Inducible Transposon Mutant Libraries in Chlamydia trachomatis.- Inducible Protein Knockdown via Engineered Small RNAs in Chlamydia.- Conditional dCas12-Mediated Gene Knockdown and Complementation in Chlamydia.- A Conditional Knockout Mutant Approach to Study Essential Genes in Chlamydia in Cell Culture and In Vivo.- Disruption of Chlamydia trachomatis Essential Genes Using DOPE (Dependence on Plasmid-Mediated Expression).- Methods for Identifying Type III Secretion System Effectors in Chlamydia trachomatis.- Split-GFP Reporter-Based Detection of Chlamydia trachomatis Inclusion Damage.- Experimental Evolution of Drug Resistance for Antibiotic Target Discovery in Chlamydia trachomatis.