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Immunophenotyping

Methods and Protocols

Gebonden Engels 2026 2e druk 9781071656068
€ 216,99
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Samenvatting

This new edition reflects the transformative developments in immunophenotyping research while maintaining a strong foundation in established methodologies essential for both research and clinical applications. Beginning with chapters involving fundamental immune cell populations, the book continues by exploring clinical applications in hematological malignancies and cellular therapies, as well as methodological advances and analytical frameworks essential for modern immunophenotyping. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Immunophenotyping: Methods and Protocols, Second Edition aims to provide researchers and clinicians with representative protocols spanning fundamental techniques to cutting-edge technologies.

Specificaties

ISBN13:9781071656068
Taal:Engels
Bindwijze:Gebonden
Aantal pagina's:484
Uitgever:Springer US
Druk:2

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Inhoudsopgave

Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T-Cells.- Enumeration of Benign and Malignant Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow Cytometric Analysis.- Neutrophil Subset Immunophenotyping.- Natural Killer Cells and Innate Lymphoid Cells Immunoprofiling.- Immunophenotyping of Human Regulatory T-Cells from Peripheral Blood Mononuclear Cells.- Immunophenotyping of Human B Lymphocytes from Peripheral Blood Mononuclear Cells.- Immunophenotyping Studies in Non-Human Primate Models.- Immunophenotypic Assessment of Natural Killer Cell Degranulation and Monocyte Function from Cryopreserved Peripheral Blood Mononuclear Cells.- Immunophenotyping of Acute Myeloid Leukemia.- Flow Cytometric Immunophenotyping of Acute Lymphoblastic Leukemia.- Clinical Flow Cytometric Testing in Chronic Lymphocytic Leukemia.- Measurable Residual Disease.- Immunophenotyping of Cellular Therapy Products Against Oncogenic and Viral Targets.- Guidelines for Gating Flow Cytometry Data for Immunological Assays.- Quality Control of Immunophenotyping.- High Immunophenotyping of Human PBMCs Using Lyophilized Antibodies.- Immunophenotyping by Single Cell CITE-Seq.- Immunophenotyping by Spatial Biology.- High Dimensional Immunophenotyping of Human Leukocytes by Spectral Cytometry.- Build Heatmaps of Cell Frequencies and Marker Intensities for Correct Interpretations.- High-Dimensional Multimodal Data Integration for Immune-Driven Diseases: A Practical Guide.

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          Immunophenotyping