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A Laboratory Course in Tissue Engineering

Gebonden Engels 2017 9781138407350
€ 308,13
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Samenvatting

Filling the need for a lab textbook in this rapidly growing field, A Laboratory Course in Tissue Engineering helps students develop hands-on experience. The book contains fifteen standalone experiments based on both classic tissue-engineering approaches and recent advances in the field. Experiments encompass a set of widely applicable techniques: cell culture, microscopy, histology, immunohistochemistry, mechanical testing, soft lithography, and common biochemical assays. In addition to teaching these specific techniques, the experiments emphasize engineering analysis, mathematical modeling, and statistical experimental design.

A Solid Foundation in Tissue Engineering and Communication Skills

Each experiment includes background information, learning objectives, an overview, safety notes, a list of materials, recipes, methods, pre- and postlab questions, and references. Emphasizing the importance for engineering students to develop strong communication skills, each experiment also contains a data analysis and reporting section that supplies a framework for succinctly documenting key results. A separate chapter provides guidelines for reporting results in the form of a technical report, journal article, extended abstract, abstract, or technical poster.

Customize Your Courses with More Than a Semester‘s Worth of Experiments

The book is a convenient source of instructional material appropriate for undergraduate or graduate students with fundamental knowledge of engineering and cell biology. All of the experiments have been extensively tested to improve the likelihood of successful data collection. In addition, to minimize lab costs, the experiments make extensive use of equipment commonly found in laboratories equipped for tissue culture. A solutions manual, available with qualifying course adoption, includes answers to pre- and postla

Specificaties

ISBN13:9781138407350
Taal:Engels
Bindwijze:Gebonden
Aantal pagina's:304
Uitgever:Taylor & Francis Ltd

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Inhoudsopgave

Getting Started in the Lab. Lab Safety. Essential Lab Skills for Tissue Engineers. Isolation of Primary Chondrocytes from Bovine Articular Cartilage. Measuring and Modeling Growth of a Cell Population. Purification of a Cell Population Using Magnetic Cell Sorting. Decellularized Matrices for Tissue Engineering. Effect of Plating Density on Cell Adhesion to Varied Culture Matrices. Dynamic versus Static Seeding of Cells onto Biomaterial Scaffolds. Cell Patterning Using Microcontact Printing. Measuring and Modeling the Motility of a Cell Population Using an Under-Agarose Assay. Characterizing Matrix Remodeling Through Collagen Gel Contraction. Effect of Substrate Stiffness on Cell Differentiation. Effect of Culture Configuration (Two versus Three Dimensions) on Matrix Accumulation. Combining In Silico and In Vitro Techniques to Engineer Pluripotent Stem Cell Fate. The Fahraeus-Lindqvist Effect: Using Microchannels to Observe Small Vessel Hemodynamics. Examining Single-Cell Mechanics Using a Microfluidic Micropipette Aspiration System. Contribution of Tissue Composition to Bone Material Properties. Technical Communication: Presenting Your Findings. Appendices. Index.

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          A Laboratory Course in Tissue Engineering