Principles and Practice of Immunoassay

The antigen-antibody reaction; production of polyclonal antibodies; production of monoclonal antibodies; modification of antibodies; separation techniques for heterogeneous immunoassays; properties of antibodies and general considerations on reagnet optimization; radio-labelled immunoassay; heterogeneous enzyme immunoassay including enzyme amplification; homogeneous enzyme mediated immunoassays including enzyme, substrate, cofactor amd inhibitor labels; fluoro-immunoassay including time resolved flourescence; homogeneous fluoro-immunoassay including fluorescence polarization and fluorescence excitatin transfer; phosporescence immunoassay; luminescence immunoassays including enhanced systems; electrometric immunoassays; alternate optical immunoassays including evanescent wave - surface plasma resonance; multilayer thin film carrier immunoassay systems; dry reagent carrier immunoassay systems; immunochromatographic immunoassays; immunoconcentration immunoassay; radial partition immunoassay; data handling for immunoassay.