Plant Cell Culture Protocols

An Introduction to Plant-Cell Culture.- Cell Culture and Plant Regeneration.- Callus Initiation, Maintenance, and Shoot Induction in Rice.- Callus Initiation, Maintenance, and Shoot Induction in Potato.- Somatic Embryogenesis in Barley Suspension Cultures.- Somatic Embryogenesis in Picea Suspension Cultures.- Direct, Cyclic Somatic Embryogenesis of Cassava for Mass Production Purposes.- Immature Inflorescence Culture of Cereals.- Cryopreservation of Rice Tissue Cultures.- Noncryogenic, Long-Term Germplasm Storage.- Plant Propagation In Vitro.- Micropropagation of Strawberry via Axillary Shoot Proliferation.- Meristem-Tip Culture for Propagation and Virus Elimination.- Clonal Propagation of Orchids.- In Vitro Propagation of Succulent Plants.- Micropropagation of Flower Bulbs.- Clonal Propagation of Woody Species.- Spore-Derived Axenic Cultures of Ferns as a Method of Propagation.- Applications for Plant Protoplasts.- Protoplast Isolation, Culture, and Plant Regeneration from Passiflora.- Isolation, Culture, and Plant Regeneration of Suspension-Derived Protoplasts of Lolium.- Protoplast Fusion for Symmetric Somatic Hybrid Production in Brassicaceae.- Production of Cybrids in Rapeseed (Brassica napus).- Microprotoplast-Mediated Chromosome Transfer (MMCT) for the Direct Production of Monosomic Addition Lines.- Guard Cell Protoplasts.- In Vitro Fertilization with Isolated Single Gametes.- Protocols for Genomic Manipulation.- Protocols for Anther and Microspore Culture of Barley.- Microspore Embryogenesis and In Vitro Pollen Maturation in Tobacco.- Embryo Rescue Following Wide Crosses.- Mutagenesis and the Selection of Resistant Mutants.- The Generation of Plastid Mutants In Vitro.- Protocols for the Introduction of Specific Genes.- Agrobacterium-Mediated Transformation of Petunia Leaf Disks.- Transformation of Rice via PEG-Mediated DNA Uptake into Protoplasts.- Transformation of Wheat via Particle Bombardment.- Plant Transformation via Protoplast Electroporation.- Transformation of Maize via Tissue Electroporation.- Transformation of Maize Using Silicon Carbide Whiskers.- Suspension Culture Initiation and the Accumulation of Metabolites.- Directing Anthraquinone Accumulation via Manipulation of Morinda Suspension Cultures.- Alkaloid Accumulation in Catharanthus roseus Suspension Cultures.- Betalains.