Plant Cell Expansion
Methods and Protocols
Gebonden Engels 2014 2015e druk 9781493919017Samenvatting
This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Chapters focus on the cutting-edge methods to study in detail several complex aspects of cell expansion such as secretion, endocytosis and recycling, cellular signaling and trafficking, and protein and polysaccharides cell wall biosynthesis in real time during cell expansion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Cell Expansion: Methods and Protocols is an essential reference book for plant scientist, molecular, and cell biologist as well as plant biochemists.
Specificaties
Lezersrecensies
Inhoudsopgave
Maja G. Rydahl, Jonatan U. Fangel, Maria Dalgaard Mikkelsen, I. Elisabeth Johansen, Amanda Andreas, Jesper Harholt, Peter Ulvskov, Bodil Jørgensen, David S. Domozych, and William G.T. Willats
2. Using Chemical Genomics to Study Cell Wall Formation and Cell Growth in Arabidopsis thaliana and Penium margaritaceum
N. Worden, V. Esteva Esteve, D. S. Domozych, and G. Drakakaki
3. Optimized Method For Growing In Vitro arabidopsis Thaliana Pollen Tubes
Cecilia Borassi, Juliana Perez Di Giorgio, María R. Scarpin, Jorge Muschietti, and Jose Estevez
4. Imaging of Calcium Dynamics in Pollen Tube Cytoplasm
María Laura Barberini and Jorge Muschietti
5. Live Imaging of Root Hairs
Silvia M. Velasquez, Jose R. Dinneny, and José M. Estevez
6. Improved ROS Measurement In Root Hair Cells
Paola Silvina Denita Juarez, Silvina Mangano, adn Jose M. Estevez
7. A Root Hair Assay to Expedite Cell Death Research
Joanna Kacprzyk and Paul F. McCabe
8. Vacuolar Staining Methods in Plant Cells
David Scheuring, Maria Schöller, Jürgen Kleine-Vehn, and Christian Löfke
9. Live Cell Imaging Of FM4-64, A Tool For Tracing The Endocytic Pathways In Arabidopsis Root Cells
Adeline Rigal, Siamsa M. Doyle, and Stéphanie Robert
10. Salt-Stress Regulation Of Root System Growth And Architecture In Arabidopsis Seedlings
Jose R. Dinneny
11. Quantification Of Fluorescent Reporters in Plant Cells
Michael Pound, Andrew P. French, and Darren M. Wells
12. Live Cell Imaging Of The Cytoskeleton And Cell Wall Enzymes In Plant Cells
Arun Sampathkumar and Raymond Wightman
13. Using the Split-Ubiquitin Yeast Two-Hybrid System to Test Protein-Protein Interactions of Transmembrane Proteins
Logan Bashline and Ying Gu
14. Activation Tag Screening For Cell Expansion Genes in Arabidopsis thaliana
Chaowen Xiao and Charles T. Anderson
15. Bifc For Protein-Protein Interactions and Protein Topology: Discussing An Integrative Approach For An Old Technique
Giovanni Stefano, Luciana Renna, and Federica Brandizzi
16. N-Glycosylation and Plant Cell Growth
Christiane Veit, Ulrike Vavra, and Richard Strasser
17. Peptide Separation Methodologies for In-depth Proteomics
Sajad Majeed Zargar, Rie Kurata, Randeep Rakwal, and Yoichiro Fukao
18. Structural And Mechanical Characterization Of Growing Arabidopsis Plant Cell Walls
Friederike Saxe, Ingo Burgert, and Michaela Eder
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