Somatic Genome Manipulation

<p>Table of Contents</p><p> </p><p>Preface for Somatic Genome Manipulation: Advances, Methods and Applications</p><p>Xiu-Qing Li, Ph.D., Research Scientist of Molecular Genetics, Agriculture and Agri-Food Canada, xiu-qing.li@agr.gc.ca; somaticgenome@gmail.com.</p><p>Danielle Donnelly, Ph.D., Associate Professor, Plant Science Dept., McGill University, danielle.donnelly@mcgill.ca. </p><p>Thomas G. Jensen, M.D., D.M.Sc., Professor of Medical Genetics and Head of the Department of Biomedicine, Aarhus University, Denmark, thomas@hum-gen.au.dk.</p><p> </p><p>Section I. Humans and animals</p><p> </p><p>Chapter 1. Drug and Gene Electrotransfer in Cancer Therapy. (Dr. Julie Gehl, M.D., Dr. Med. Sci., Consultant Oncology </p><p>C*EDGE (Center for Experimental Drug and Gene Electrotransfer), Department of Oncology, Copenhagen University Hospital Herlev, Herlev Ringvej 75, 2730 Herlev, Denmark, E-mail: Julie.Gehl@regionh.dk.</p><p> </p><p>Chapter 2. Targeted Porcine Genome Engineering with TALENs. Yonglun Luo^{*, 1}; Lin Lin¹; Monika M. Golas ^{1, 2};  Charlotte Brandt Sørensen^{1, 3};  and Lars Bolund¹; </p><p>¹Department of Biomedicine, Aarhus University, Aarhus C, 8000, Denmark, Yonglun ALUN@HUM-GEN.AU.DK </p><p>²Center for Stochastic Geometry and Advanced Bioimaging, Aarhus University, Aarhus C, 8000, Denmark</p><p>³Department of Clinical Medicine - The Department of Cardiological Medicine B, Aarhus University, Brendstrupgårdsvej 100, 8200 Aarhus N, Danmark</p><p> </p><p>Chapter 3. Somatic Gene Therapy using Viral Vectors: theoretical and clinical implications in relation to treatment of genetic conditions in humans. Anne Louise Askou and Thomas J. Corydon* Department of Biomedicine, Faculty of Health, Aarhus University, Denmark *Corresponding author: TJC, Department of Biomedicine, Faculty of Health, Wilhelm Meyers Allé 4, Aarhus University, 8000 Aarhus C, Denmark, Phone: (+45) 28992179; E-mail: tjc@hum-gen.au.dk.</p><p> </p><p>Chapter 4. Nonviral Gene Therapy – The Challenge of Mobilizing DNA. Jacob Giehm Mikkelsen* MD, *Corresponding author. Department of Biomedicine, Aarhus University, Wilh. Meyers Allé 4, DK-8000 Aarhus C, Denmark. Phone: +45 87167767. Fax: +45 86123173. Email: giehm@hum-gen.au.dk.</p><p> </p><p>Chapter 5. Human Stromal Stem Cell Therapy Using Gene Modified Cells</p><p>Walid Zaher^{1, 3} and Moustapha Kassem^{1, 2, 3}: ¹Department of Endocrinology and Metabolism, Endocrine Research Laboratory (KMEB), Odense University Hospital &amp; University of Southern Denmark, Odense, Denmark. ²Danish Stem Cell Center (DanStem), Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. ³Stem Cell Unit, Department of Anatomy, College of Medicine, King Saud University, Saudi Arabia. </p><p>* Corresponding author. Moustapha Kassem, professor, Endocrine Research Laboratory (KMEB), Department of Endocrinology, Odense University Hospital, Kloevervaenget 6, 4th floor, DK-5000 Odense C, Denmark. Tel: 45-65504084, 45-65411606; Fax: +66503920; E-mail: mkassem@health.sdu.dk</p><p> </p><p>Chapter 6. Somatic Cell Nuclear Transfer and the Creation of Transgenic Large Animal Models.  Naomi Dicks, Luis B. Agellon* and Vilceu Bordignon*, *Corresponding authors. Mailing address: </p><p>Vilceu Bordignon, McGill University, Department of Animal Science, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada. Phone: 514-398-7793. Fax: 514-398-7964.  E-mail: vilceu.bordignon@mcgill.ca</p><p>Luis B. Agellon, McGill University, School of Dietetics and Human Nutrition, 21,111 Lakeshore Road, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada. Phone: 514-398-7862. Fax: 514-398-7739. E-mail: luis.agellon@mcgill.ca</p><p> </p><p> </p><p>Section II. Plants </p><p> </p><p>Chapter 7. Apomixis: The Asexual Formation of Seed. Ross Bicknell* and Andrew Catanach, Plant and Food Research, Private Bag 4704, Christchurch, New Zealand</p><p>*Corresponding author Tel: 64-3-325-9445, Fax 64-3-315-2074, Email ross.bicknell@plantandfood.co.nz</p><p> </p><p>Chapter 8. Somatic Embryogenesis for Potato (Solanum tuberosum L.) Improvement.</p><p>Nassar^1,2,3, A.M.K., S. Kubow², and D.J. Donnelly¹</p><p>¹Plant Science Department, ²School of Dietetics &amp; Human Nutrition, McGill University, 21,111 Lakeshore Rd., Sainte Anne de Bellevue, QC, Canada H9X 3V9, ³Pest Control and Environmental Protection Department, Faculty of Agriculture, Damanhour University, Al-Gomhouria Street, Damanhour, Albeheira, Egypt PO Box 22516;</p><p>danielle.donnelly@mcgill.ca</p><p> </p><p>Chapter 9. Brassica Ogu-INRA Cytoplasmic Male Sterility: An Example of Successful Plant Somatic Fusion for Hybrid Seed Production. G. Pelletier* &amp; F. Budar, Institut Jean-Pierre Bourgin, INRA-AgroParisTech UMR 1318, Versailles France.</p><p>*Corresponding author: G. Pelletier</p><p>Institut Jean-Pierre Bourgin, INRA-AgroParisTech UMR 1318, Versailles France. </p><p>Phone: +33 130 833 317, Fax: +33 130 833 319, E-mail: Georges.Pelletier@versailles.inra.fr</p><p> </p><p>Chapter 10. Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusion. Veli-Matti Rokka, PhD, MTT Agrifood Research Finland, Biotechnology and Food Research, Myllytie 1, FI-31600 Jokioinen, Finland, e-mail: veli-matti.rokka@mtt.fi, telephone: +358 29 5317685</p><p> </p><p>Chapter 11. Strategic RNA Silencing for Plant Viral Resistance</p><p>Jin Ma^1,2, Changxiang Zhu³, Fujiang Wen³ and Xiu-Qing Li¹</p><p>¹Forestry and Environmental Management, University of New Brunswick, Fredericton, New Brunswick, E3B 6C2, Canada; ²Potato Research Center, Agriculture and Agri-Food Canada, P.O. Box 20280, Fredericton, New Brunswick, E3B 4Z7, Canada;³State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, 271018, P. R. China</p><p> </p><p>Chapter 12. Targeted Gene Mutation in Plants</p><p>Kunling Chen and Caixia Gao * </p><p>State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.</p><p>*Corresponding author. Mailing address: State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Phone:00861064807727; Fax: 00861064807727; E-mail: cxgao@genetics.ac.cn.</p><p> </p><p>Section III. General experimental and bioinformatic technologies</p><p> </p><p>Chapter 13. Mitochondrial Genetic Manipulation. </p><p>Daria Mileshina, Adnan Khan Niazi, Frédérique Weber-Lotfi, José Gualberto and André Dietrich*</p><p>Institut de Biologie Moléculaire des Plantes, CNRS and Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg, France</p><p>*Corresponding author. Mailing address: Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084 Strasbourg, France. Phone: +33 3 67 15 53 73. Fax: +33 3 88 61 44 42. E-mail: andre.dietrich@ibmp-cnrs.unistra.fr</p><p> </p><p>Chapter 14.  Laboratory Methods for Investigating Nuclear and Cytoplasmic Genomes and Transcriptome.  Xiu-Qing Li, PhD (Docteur d’Etat of France) Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Fredericton, NB, Canada E3B 4Z7; e-mail: Xiu-Qing.Li@agr.gc.ca.</p><p> </p><p>Chapter 15. Bioinformatic approaches for analysis of gene direction, chromosome base composition, mRNA polyadenylation, and protein network. Xiu-Qing Li, PhD, Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Fredericton, NB, Canada E3B 4Z7; e-mail: Xiu-Qing.Li@agr.gc.ca</p>