HIV Protocols

<p>Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System.- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry.- HIV-1 Capsid Stabilization Assay.- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity.- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations.- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector.- Novel Biochemical Tools for Probing HIV RNA Structure.- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq.- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations.- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase.- Quantification of HIV-1 Gag Localization Within Virus Producer Cells.- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro.- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy.- Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice.- High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis.- Measuring the Frequency of Latent HIV-1 in Resting CD4⁺ T Cells Using a Limiting Dilution Co-Culture Assay.- LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds.- Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies.- Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells.- The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients.- Proteomic Characterization of Exosomes from HIV-1-Infected Cells.- Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot.- Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay.- Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up.- Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.</p>