Nonribosomal Peptide and Polyketide Biosynthesis

Part I: Background and Overview

 

1. Structural Biology of Nonribosomal Peptide Synthetases

            Bradley R. Miller and Andrew M. Gulick

 

2. The Assembly Line Enzymology of Polyketide Biosynthesis

            Marisa Till and Paul R. Race

 

Part II: In Vitro Methods

 

3. Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay

            Benjamin P. Duckworth, Daniel J. Wilson, and Courtney C. Aldrich

 

4. Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains

            Fumihiro Ishikawa and Hideaki Kakeya

 

5. Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases

            Chitose Maruyama, Haruka Niikura, Masahiro Takakuwa, Hajime Katano, and Yoshimitsu Hamano

 

6. Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis

            Clara Brieke, Veronika Kratzig, Madeleine Peschke, and Max J. Cryle

 

7. Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro

            Ralph A. Cacho and Yi Tang

 

8. The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis

            Haoran Zhang, Lei Fang, Marcia S. Osburne, and Blaine A. Pfeifer

 

9. Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC‒MS/MS-Based Proteomics

            Yunqiu Chen, Ryan A. McClure, and Neil L. Kelleher

 

10. Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi

            Alexandra A. Soukup, Nancy P. Keller, and Philipp Wiemann

 

11. In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

            Delphine Debois, Marc Ongena, Hélène Cawoy, and Edwin De Pauw

 

12. Secondary Metabolic Pathway-Targeted Metabolomics

            Maria I. Vizcaino and Jason M. Crawford

 

Part III: Bioinformatic Methods

 

13. Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra

 

14. Bioinformatics Tools for the Discovery of New Nonribosomal Peptides

            Valérie Leclère, Tilmann Weber, Philippe Jacques, and Maude Pupin

 

15. The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways

            Nicolas Tremblay, Patrick Hill, Kyle R. Conway, and Christopher N. Boddy

 

16. Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains

            Guillermin Agüero-Chapin, Gisselle Pérez-Machado, Aminael Sánchez-Rodríguez, Miguel Machado Santos, and Agostinho Antunes

 

17. Characterization of Nonribosomal Peptide Synthetases with NRPSsp

            Carlos
Prieto