Quantitative Proteomics by Mass Spectrometry

Gebonden Engels 2016 2e druk 9781493935222
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Samenvatting

This volume describes prominent
methodologies developed by laboratories that have been leading the field of
quantitative proteomics by mass spectrometry. The procedures for performing the
experiments are described in an easy-to-understand manner with many technical
details that usually are not reported in typical research articles. This second
edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies
used for quantifying proteins and post-translational modifications in different
types of biomedical specimens. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.

Authoritative and thorough, Quantitative
Proteomics by Mass Spectrometry, Second Edition is a
valuable resource to help researchers understand and learn about the
latest tools used in the study of quantitative proteomics by mass spectrometry.

 

Specificaties

ISBN13:9781493935222
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York
Druk:2

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Inhoudsopgave

<p>1. Increased
Depth and Breadth of Plasma Protein Quantitation via Two Dimensional Liquid
Chromatography/Multiple Reaction Monitoring-Mass Spectrometry with Labeled
Peptide Standards<br>Andrew J. Percy, Juncong Yang, Andrew G. Chambers, and Christoph
H. Borchers<br><br>2. Quantitative
Analysis of the Wirt5-Regulated Lysine Succinylation Proteome in Mammalian
Cells<br>Yue Chen<br><div><br>3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Determining
the Composition and Stability of Protein Complexes Using an Integrated Label-Free
and Stable Isotope Labeling Strategy<br>Todd M. Greco, Amanda J. Guise, and Ileana M. Cristea<br><br>4.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
Label-Free
Quantitation for Clinical Proteomics<br>Robert Moulder, Young Ah Goo, and David
R. Goodlett<br><br>5.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Proteogenomic
Methods to Improve Genome Annotation<br>Keshava K. Datta, Anil K. Madugundu, and Harsha Gowda<br><br>6.&nbsp; Mass
Spectrometry-Based Quantitative O-GlcNAcomic Analysis<br>Junfeng Ma and Gerald W. Hart<br><br>7.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
Isolating and
Quantifying Plasma HDL Proteins by Sequential Density Gradient
Ultracentrifugation and Targeted Proteomics<br>Clark M. Henderson, Tomas Vaisar, and
Andrew N. Hoofnagle<br><br>8.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
A Method for
Label-Free Differential Top-Down Proteomics<br>Ioanna Ntai, Timothy K. Toby, Richard
D. LeDuc, and Neil L. Kelleher<br><br>9.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Multiplexed Immunoaffinity Enrichment of
Peptides with Anti-Peptide Antibodies and Quantification by Stable Isotope
Dilution Multiple Reaction Monitoring Mass Spectrometry<br>Eric
Kuhn and Steven A. Carr<br><br>10.&nbsp; &nbsp;High
Throughput Quantitative Proteomics Enabled by Mass Defect-Based 12-Plex DiLeu
Isobaric Tags<br>Dustin C. Frost and Lingjun Li<br><br>11.&nbsp; &nbsp;Isotopic
N, N-Dimethyl Leucine (iDiLeu) for Absolute Quantification of Peptides Using a
Standard Curve Approach<br>Tyler Greer and Lingjun Li<br><br>12.&nbsp; &nbsp;Selecting
Optimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins as
Analytical Standards<br>James G. Bollinger, Andrew B.
Stergachis, Richard S. Johnson, Jarrett D. Egertson, and Michael J. MacCoss<br><br>13.&nbsp; &nbsp;Using
the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted
Proteomics Assays<br>Jeffrey R Whiteaker, Goran N. Halusa,
Andrew N. Hoofnagle, Vagisha Sharma, Brendan MacLean, Ping Yan, John A. Wrobel,
Jacob Kennedy, DR Mani, Lisa J. Zimmerman, Matthew R. Meyer, Mehdi Mesri, Susan
E. Abbatiello, Emily Boja, Stven A. Carr, Daniel W. Chan, Xian Chen, Jing Chen,
Sherri R. Davies, Matthew J. C. Ellis, David Fenyö, Tara Hiltke, Karen A.
Ketchum, Chris Kinsinger, Eric Kuhn, Daniel C. Liebler, De Lin, Tao Liu,
Michael Loss, Michael J. MacCoss, Wei-Jun Qian, Robert Rivers, Karin D.
Rodland, Kelly V. Ruggles, Mitchell G. Scott, Richard D. Smith, Stefani Thomas,
R. Reid Townsend, Gordon Whiteley, Chaochao Wu, Hui Zhang, Zhen Zhang, Henry
Rodriguez, and Amanda G. Paulovich<br><br>14.&nbsp; &nbsp;Large-Scale
and Deep Quantitative Proteome Profiling Using Isobaric Lableing Coupled with
Two-Dimensional LC-MS/MS<br>Marina A. Gritsenko, Zhe Xu, Tao Liu,
and Richard D. Smith<br><br>15.&nbsp; &nbsp;Multiple
and Selective Reaction Monitoring Using Triple Quadrupole Mass Spectrometer:
Pre-Clinical Large Cohort Analysis<br>Qin Fu, Zhaohui Chen, Shenyan Zhang,
Sarah Parker, Zongming Fu, Xiaoqian&nbsp; Liu,
Josef Coresh, and Jennifer E. Van Eyk<br><br>16.&nbsp; &nbsp;Methods
for SWATH™: Data Independent Acquisition on TripleTOF Mass Spectrometers<br>Ronald J. Holewinski, Sarah J.
Parker, Andrea D. Matlock, Vidya Venkatraman, and Jennifer E. Van Eyk<br><br>17.&nbsp; &nbsp;Measurement
of Phosphorylated Peptides with Absolute Quantification<br>Raven J. Reddy,<sup> </sup>Timothy G.
Curran, Yi Zhang, and Forest M. White<br><br><div></div></div></p><div>18.&nbsp;&nbsp;&nbsp;&nbsp;
&nbsp;Proteomic Analysis of Protein Turnover by Metabolic Whole Rodent Pulse-Chase Isotopic Labeling and Shotgun Mass Spectrometry Analysis<br></div>Jeffrey N. Savas, Sung Kyu Park, and John
R. Yates III<p></p>

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