Angiogenesis Protocols
Gebonden Engels 2016 3e druk 9781493936267Samenvatting
In
addition to updating important core techniques this third edition presents new
chapters on assessing leukocyte involvement in angiogenesis, lymphatic cell and
pericyte isolation techniques, spheroid and arterial ring based in vitro
assays, and on pericyte involvement in angiogenesis. Angiogenesis Protocols,
Third Edition also guides readers through new protocols on chorioallantoic
membrane models, corneal pocket assays to assess angio- and lymphangiogenesis,
models of muscle angiogenesis, and use of zebrafish embryos to study vascular
angiogenesis and senescence. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative
and practical, Angiogenesis Protocols, Third Edition provides not only a
practical handbook for key techniques, but also an informative and enjoyable
read for all those interested, no matter how directly, in angiogenesis.
Specificaties
Lezersrecensies
Inhoudsopgave
Methods for Measuring Tissue Lymphangiogenesis.- Immunohistochemical Assessment
of Leukocyte Involvement in Angiogenesis.- Isolation and Culture of Human
Endothelial Cells from Micro- And Macro-Vessels.- Isolation, Identification and
Culture of Human Lymphatic Endothelial Cells.- Isolation, Culture and
Characterisation Of Vascular Smooth Muscle Cells.- Isolation and Transfection of
Primary Culture Bovine Retinal Pericytes.- In Vitro Assays for Endothelial Cell
Functions Required for Angiogenesis: Proliferation, Motility, Tubular
Differentiation And Matrix Proteolysis.- Tube Forming Assays.- In Vitro
Co-Culture Assays of Angiogenesis.- Spheroid-Based In Vitro Angiogenesis Model.-
Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel
In Vitro Model for the Study Of Angiogenesis.- Human Arterial Ring Angiogenesis
Assay.- A Modified Aortic Ring Assay to Assess Angiogenic Potential InVitro.- Quantitative
Imaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics and
Angiogenesis.- Static and Dynamic Assays of Cell Adhesion Relevant to the
Vasculature.- Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis
by Intravital Microscopy.- Quantitative Estimation of Tissue Blood Flow Rate.- Chorioallantoic
Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis,
Vascular Permeability, and Tumor Cell Intravasation.- The Rabbit Corneal Pocket
Assay.- The Corneal Micropocket Assay: A
Model of Angiogenesis and Lymphangiogenesis.- Models of Oxygen Induced
Retinopathy in Rodents.- The Sponge Implant Model of Angiogenesis.- Measurement
of Angiogenesis, Arteriolargenesis and Lymphangiogenesis Phenotypes by Use of
Two-Dimensional Mesenteric Angiogenesis Assay.- In Vivo Models of Muscle
Angiogenes.- Use of the Hollow Fibre Assay to Evaluate Agents that Target The
Tumor Neovasculature.- Angiogenesis and Senescence in Zebrafish Embryos. </p>
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