Type-1 Diabetes

Methods and Protocols

Gebonden Engels 2016 9781493936410
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Specificaties

ISBN13:9781493936410
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York

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Inhoudsopgave

<p>Type 1 Diabetes: Prediction And Prevention: Current Perspectives.-&nbsp;The HLA.-&nbsp;Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.-&nbsp;Islet Autoantibody Analysis: Radioimmunoassays.-&nbsp;Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.-&nbsp;Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.-&nbsp;Histology of the Type 1 Diabetes Pancreas.-&nbsp;Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.-&nbsp;Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.-&nbsp;Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.-&nbsp;Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.-&nbsp;FISH with Concomitant Immunofluorescence.-&nbsp;Laser Capture Microdissection:&nbsp; Single Pancreatic Cell DNA Profiling.-&nbsp;The Gut Microbiome in the NOD Mouse.-&nbsp;Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes.&nbsp;</p>

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        Type-1 Diabetes