Molecular Genetics of Asthma

<p>Applications of Molecular Genetics to the Study of Asthma.-&nbsp;Assessment and Validation of New Genetic Variants: A systematic In Silico Approach.-&nbsp;Introduction to the Gene Expression Analysis.-&nbsp;Real Time PCR for Gene Expression Quantification in Asthma.-&nbsp;Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.-&nbsp;Review of Methods to Study Gene Expression Regulation Applied to Asthma.-&nbsp;Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.-&nbsp;SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma.-&nbsp;Chromatin Immunoprecipitation: Application to the Study of Asthma.-&nbsp;Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols.-&nbsp;Protocols for Exosome Isolation and RNA Profiling.-&nbsp;Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell.-&nbsp;Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line.-&nbsp;Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.-&nbsp;Review of Mouse Models Applied to the Study of Asthma.-&nbsp;General and Specific Mouse Models for Asthma Research.-&nbsp;Mouse Models applied to the Research of Pharmacological Treatments in Asthma.-&nbsp;Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.</p>