Pseudomonas Methods and Protocols

<p>Gene Transfer: Transduction.- Gene Transfer: Transformation/Electroporation.- Gene transfer: Conjugation.- Pseudomonas Bacteriophage Isolation and Production.- Genotyping Methods.- Drug Susceptibility Testing by Dilution Methods.- Plate-based Assay for Swimming Motility in Pseudomonas aeruginosa.- Plate-based Assay for Swarming Motility in Pseudomonas aeruginosa.- Motility Assay: Twitching Motility.- Qualitative and Quantitative Assays for Flagellum-mediated Chemotaxis.- Microscopic Analysis: Morphotypes and Cellular Appendages.- Determination of Lipolytic Activities.- Elastinolytic and proteolytic enzymes.- In vitro Assays to Monitor the Activity of Pseudomonas aeruginosa Type III Secreted Proteins.- Cell Fractionation.- Characterization of Molecular Interactions using Isothermal Titration Calorimetry.- Proteomic Analysis.- Membrane Proteomics of Pseudomonas aeruginosa.- Construction of Pseudomonas Two-hybrid Libraries for High-throughput Assays.- Biosensors for Qualitative and Semi-quantitative Analysis of Quorum Sensing Signal Molecules.- LC-MS/MS Quantitative Analysis of Quorum Sensing Signal Molecules.- LC/MS/MS-Based Quantitative Assay for the Secondary Messenger Molecule, c-di-GMP.- Metabolic Footprinting: Extracellular Metabolomic Analysis.- Pyoverdine and Pyochelin Measurements.- Measurement of Phenazines in Bacterial Cultures.- Extraction and Measurement of NAD(P)⁺ and NAD(P)H.- Cyanide Measurements in Bacterial Culture and Sputum.- Monitoring Iron Uptake by Siderophores.- Exopolysaccharide Quantification.- Liquid Chromatography/Mass Spectrometry for the Identification and Quantification of Rhamnolipids.- LPS quantitation Procedures.- Monitoring Lectin Interaction with Carbohydrates.- Mining the Pseudomonas Genome.- Identification of Bacterial Small RNAs by RNA Sequencing.- Gene Amplification and qRT-PCR.- The Standard European Vector architecture (SEVA) plasmid toolkit.- Chromosomal integration of transcriptional fusions.- A Method toCapture Large DNA Fragments from Genomic DNA.- Transposon Mutagenesis.- Site-directed Mutagenesis and Gene Deletion using Reverse Genetics.- Signature Tagged Mutagenesis.- Construction of a Pseudomonas aeruginosa Genomic Bank.- Strategy for Genome Sequencing Analysis and Assembly for Comparative Genomics of Pseudomonas Genomes.- Promoter Fusions with Optical Outputs in Individual Cells and in Populations.- Chromatin Immunoprecipitation for ChIP-chip and ChIP-Seq.- Transcriptional Analysis of Pseudomonas aeruginosa Infected Caenorhabditis elegans.- Methods for Studying Biofilm Formation: Flow Cells and Confocal Laser Scanning Microscop.- Biofilm Formation in the 96-well Microtiter Plate.- Methods for Studying Biofilm Dispersal in Pseudomonas aeruginosa.- Pseudomonas aeruginosa PA14 Pathogenesis in Caenorhabditis Elegans.- Assessing Pseudomonas Virulence using a Non-mammalian Host Model: Dictyostelium Discoideum.- Assessing Pseudomonas virulence with non-mammalian host: Galleria mellonella.- Assessing Pseudomonas Virulence with the Non-mammalian Host: Arabidopsis Thaliana.- Assessing Pseudomonas aeruginosa Persister/Antibiotic Tolerant Cells.- Assessing Pseudomonas Virulence with Non-mammalian Host: Zebrafish.- Assessing Pseudomonas Virulence with a Non-mammalian Host: Drosophila Melanogaster.- Assessing Pseudomonas Virulence using Host Cells.- Assessing Pseudomonas aeruginosa Virulence and the Host Response using Murine Models of Acute and Chronic Lung Infection.- Assessing Pseudomonas Virulence using Mammalian Models: Acute Infection Model.- Burn Mouse Models.</p>