Structural Genomics and Drug Discovery

Data Management in the Modern Structural Biology and Biomedical Research Environment.- Structural Genomics of Human Proteins.- Target Selection for Structural Genomics of Infectious Diseases.- Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery.- High Throughput Cloning for Biophysical Applications.- Expression and Solubility Testing in a High Throughput Environment.- Protein Production for Structural Genomics Using E. coli Expression.- Eukaryotic Expression Systems for Structural Studies.- Automated Cell-free Protein Production Methods for Structural Studies.- Parallel Protein Purification.- Oxidative Refolding from Inclusion Bodies.- High throughput Crystallization Screening.- Screening Proteins for NMR Suitability.- Salvage or Recovery of Failed Targets by in situ Proteolysis.- Salvage of Failed Protein Targets by Reductive Alkylation.- Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy.- Data Collection for Crystallographic Structure Determination.- Structure, Determination, Refinement, and Validation.- Virtual High-Throughput Ligand Screening.- Ligand Screening using Fluorescence Thermal Shift Analysis (FTS).- Ligand Screening using Enzymatic Assays.- Ligand Screening using NMR.- Screening Ligands by X-ray Crystallography.- Case Study – Structural Genomics and Human Protein Kinases.