Tissue Morphogenesis

Methods and Protocols

Paperback Engels 2017 9781493945856
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

Tissue Morphogenesis: Methods and Protocols highlights major techniques, both experimental and computational, for the study of tissue morphogenesis, divided into several sections, with specific focus on techniques to image, manipulate, model and analyze tissue morphogenesis. Chapters focus on imagining analysis of tissue morphogenesis, culture models of tissue morphogenesis, manipulating cells and tissues in vivo, novel model systems to investigate issue morphogenesis and computational models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Tissue Morphogenesis: Methods and Protocols serves as a primary resource for both fundamental and practical understanding of the techniques used to uncover the basis of tissue morphogenesis.

Specificaties

ISBN13:9781493945856
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

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Inhoudsopgave

<p>PART I          IMAGING MORPHOGENESIS</p><p> Chapter 1.       Probing Regional Mechanical Properties of Embryonic Tissue Using                                                Microindentation and Optical Coherence Tomography</p><p>Benjamin A. Filas, Gang Xu, and Larry A. Taber</p><p> </p><p>Chapter 2.       Hemodynamic Flow Visualization of Early Embryonic Great Vessels using mPIV</p><p>Selda Goktas, Chia-Yuan Chen, William J. Kowalski, and Kerem Pekkan</p><p> </p><p>Chapter 3.       Using Correlative Light and Electron Microscopy to Study Zebrafish Vascular Morphogenesis</p><p>Jacky G. Goetz, Fabien Monduc, Yannick Schwab, and Julien Vermot</p><p> </p><p>Chapter 4.       Micro/Nano-Computed Tomography Technology for Quantitative Dynamic, Multi-scale Imaging of Morphogenesis</p><p> Chelsea L. Gregg, Andrew K. Recknagel, and Jonathan T. Butcher</p><p> </p><p>Chapter 5.       Imaging the Dorsal-Ventral Axis of Live and Fixed Drosophila Melanogaster Embryos</p><p>Sophia N. Carrell and Gregory T. Reeves</p><p> </p><p>Chapter 6.       Light-Sheet-Based Imaging and Analysis of Early Embryogenesis in the Fruit Fly</p><p>Khaled Khairy, William Lemon, Fernando Amat, and Philipp J. Keller</p><p> </p><p>Chapter 7.       Quantitative Image Analysis of Cell Behavior and Molecular Dynamics During Tissue Morphogenesis</p><p> Chun Yin Bosco Leung and Rodrigo Fernandez-Gonzalez</p><p> </p><p>Chapter 8.       A Multiplex Fluorescent in situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis</p><p> Lily S. Cheung and Stanislav Shvartsman</p><p> </p><p>Chapter 9.       Active Cell and ECM Movements During Development</p><p>Anastasiia Aleksandrova, Brenda J. Rongish, Charles D. Little, and Andras Czirok</p><p> </p><p>PART II         CULTURE MODELS OF MORPHOGENESIS</p><p> Chapter 10.     3D Culture Assays of Murine Mammary Branching Morphogenesis and Epithelial Invasion</p><p>Kim-Vy Nguyen-Ngoc, Eliah R. Shamir, Robert J. Huebner, Jennifer N. Beck, Kevin J. Cheung, and Andrew J. Ewald</p><p> Chapter 11.     Culture of Mouse Embryonic Foregut Explants</p><p> Felicia Chen and Wellington V. Cardoso</p><p> Chapter 12.     Investigating Human Vascular Tube Morphogenesis and maturation using  endothelial cell-pericyte co-cultures and a doxycycline-inducible genetic system in 3D extracellular matrices</p><p> Stephanie L.K. Bowers, Chun-Xia Meng, Matthew T. Davis, and George E. Davis</p><p> Chapter 13.     Three-Dimensional Traction Force Microscopy of Engineered Epithelial Tissues</p><p> Alexandra S. Piotrowski, Victor D. Varner, Nikolce Gjorevski, and Celeste M. Nelson</p><p> </p><p>PART III       MANIPULATING CELLS AND TISSUES IN VIVO</p><p>Chapter 14.     Probing Cell Mechanics with Sub-Cellular Laser Dissection of Actomyosin  Networks in the Early Developing Drosophila Embryo</p><p> M. Rauzi and P.-F. Lenne</p><p> </p><p>Chapter 15.     UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish    Embryo in vivo and ex vivo</p><p>Michael Smutny, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp Heisenberg</p><p> </p><p>Chapter 16.     Measurement of Intercellular Cohesion by Tissue Surface Tension</p><p> Ramsey A. Foty</p><p> </p><p>Chapter 17.     Quail-Chick Chimeras and Eye Development</p><p> Sinu Jasrapuria Agrawal and Peter Y. Lwigale</p><p> </p><p>PART IV       EMERGING MODELS OF TISSUE MORPHOGENESIS</p><p> Chapter 18.     Studying Epithelial Morphogenesis in Dictyostelium</p><p> Daniel J. Dickinson, W. James Nelson, and William I. Weis</p><p> </p><p>Chapter 19.     Primary Cell Cultures of Regenerating Holothurian Tissues</p><p> Samir A. Bello, Ricardo J. Abreu-Irizarry, and Jose E. Garcia-Arraras</p><p> </p><p>PART V         COMPUTATIONAL MODELS OF TISSUE MORPHOGENESIS</p><p> Chapter 20.     Large-Scale Parameter Studies of Cell-Based Models of Tissue Morphogenesis   Using CompuCell3D or VirtualLeaf</p><p>Margriet M. Palm and Roeland M. H. Merks</p><p> </p><p>Chapter 21.     Simulating Tissue Morphogenesis and Signaling</p><p>Dagmar Iber, Simon Tanaka, Patrick Fried, Philipp Germann, and Denis  Menshykau</p><p> </p><p>Chapter 22.     Elasticity-Based Targeted Growth Models of Morphogenesis</p><p> Patrick W. Alford</p>

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