Polyadenylation in Plants

Computational Analysis Of Plant Polyadenylation Signals.- Prediction of Plant mRNA Polyadenylation Sites.- Extraction of poly(A) Sites from Large-scale RNA-seq Data.- Poly(A)-tag Deep Sequencing Data Processing To Extract poly(A) Sites.- Analysis of poly(A) Site Choice Using A Java-Based Clustering Algorithm.- RADPRE: a Computational Program for Identification of Differential mRNA Processing Including Alternative Polyadenylation.- Characterization Of Plant Polyadenylation Complexes By Using Tandem Affinity Purification.- In vitro Analysis Of Cleavage And Polyadenylation In Arabidopsis.- Production, Purification, and Assay of Recombinant Proteins for in vitro Biochemical Analyses of the Plant Polyadenylation Complex.- Detection of Disulfide Linkage By Chemical Derivatization And Mass Spectrometry.- Transient Expression Using Agroinfiltration To Study Polyadenylation In Plants.- A 3’ RACE Protocol To Confirm Polyadenylation Sites.- Phage Display Library Screening for Identification of Interacting Protein Partners.- Genome-wide Determination Of Poly(A) Site Choice In Plants.- DNA/RNA Hybrid Primer Mediated Poly(A) Tag Library Construction for Illumina Sequencing.- Poly(A) Tag Library Construction from 10 ng Total RNA.- Genome-Wide Analysis Of Distribution of RNA Polymerase II isoforms using ChIP-seq.